Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene
| Autor: | Bernhard Dietzschold, R I Macfarlan, William H. Wunner, Marie-Paule Kieny, Tadeusz J. Wiktor, J P Lecocq, Richard Lathe, M Mackett, P J Curtis, K J Reagan |
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| Rok vydání: | 1984 |
| Předmět: |
Genes
Viral viruses Genetic Vectors DNA Recombinant Mokola virus Vaccinia virus Biology Recombinant virus medicine.disease_cause Virus Mice Viral Proteins chemistry.chemical_compound medicine Animals Glycoproteins Duck embryo vaccine Immunity Cellular Multidisciplinary Rabies virus medicine.disease biology.organism_classification Virology Rabies Vaccines chemistry Duvenhage virus Female Rabies Vaccinia Immunologic Memory T-Lymphocytes Cytotoxic Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 81:7194-7198 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.81.22.7194 |
| Popis: | Inoculation of rabbits and mice with a vaccinia-rabies glycoprotein recombinant (V-RG) virus resulted in rapid induction of high concentrations of rabies virus-neutralizing antibodies and protection from severe intracerebral challenge with several strains of rabies virus. Protection from virus challenge also was achieved against the rabies-related Duvenhage virus but not against the Mokola virus. Effective immunization by V-RG depended on the expression of a rabies glycoprotein that registered proline rather than leucine as the eighth amino acid from its NH2 terminus (V-RGpro8). A minimum dose required for effective immunization of mice was 10(4) plaque-forming units of V-RGpro8 virus. beta-propiolactone-inactivated preparations of V-RGpro8 virus also induced high levels of rabies virus-neutralizing antibody and protected mice against intracerebral challenge with street rabies virus. V-RGpro8 virus was highly effective in priming mice to generate a secondary rabies virus-specific cytotoxic T-lymphocyte response following culture of lymphocytes with either ERA or PM strains of rabies virus. |
| Databáze: | OpenAIRE |
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