Analysis of a Preliminary microRNA Expression Signature in a Human Telangiectatic Osteogenic Sarcoma Cancer Cell Line

Autor: Palmini, G., Romagnoli, C., Donati, S., Zonefrati, R., Galli, G., Marini, F., Lantomasi, T., Aldinucci, A., Leoncini, G., Franchi, A., Beltrami, G., Campanacci, D. A., Capanna, R., Brandi, M. l. Capanna R.
Rok vydání: 2020
Předmět:
0301 basic medicine
cancer stem cells
Biopsy
Fluorescent Antibody Technique
Bone Neoplasms
Biology
medicine.disease_cause
Catalysis
Article
Metastasis
Inorganic Chemistry
lcsh:Chemistry
03 medical and health sciences
0302 clinical medicine
Cancer stem cells
microRNAs
Telangiectatic osteosarcoma
Biomarkers
Cell Line
Tumor
Humans
Immunohistochemistry
MicroRNAs
Osteosarcoma
Gene Expression Profiling
Gene Expression Regulation
Neoplastic
Transcriptome
telangiectatic osteosarcoma
Cancer stem cell
microRNA
medicine
Physical and Theoretical Chemistry
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Organic Chemistry
General Medicine
medicine.disease
Computer Science Applications
Gene expression profiling
030104 developmental biology
Telangiectatic Osteosarcoma
lcsh:Biology (General)
lcsh:QD1-999
Tumor progression
030220 oncology & carcinogenesis
Cancer research
Carcinogenesis
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 1163, p 1163 (2021)
Volume 22
Issue 3
ISSN: 1422-0067
Popis: Telangiectatic osteosarcoma (TOS) is an aggressive variant of osteosarcoma (OS) with distinctive radiographic, gross, microscopic features, and prognostic implications. Despite several studies on OS, we are still far from understanding the molecular mechanisms of TOS. In recent years, many studies have demonstrated not only that microRNAs (miRNAs) are involved in OS tumorigenesis, development, and metastasis, but also that the presence in high-grade types of OS of cancer stem cells (CSCs) plays an important role in tumor progression. Despite these findings, nothing has been described previously about the expression of miRNAs and the presence of CSCs in human TOS. Therefore, we have isolated/characterized a putative CSC cell line from human TOS (TOS-CSCs) and evaluated the expression levels of several miRNAs in TOS-CSCs using real-time quantitative assays. We show, for the first time, the existence of CSCs in human TOS, highlighting the in vitro establishment of this unique stabilized cell line and an identification of a preliminary expression of the miRNA profile, characteristic of TOS-CSCs. These findings represent an important step in the study of the biology of one of the most aggressive variants of OS and the role of miRNAs in TOS-CSC behavior.
Databáze: OpenAIRE
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