Development of a multiplex DNA-based traceability tool for crop plant materials
| Autor: | Ralf Seyfarth, Esther J. Kok, Marleen M. Voorhuijzen, Theo W. Prins, Jeroen P. van Dijk, AM Angeline Van Hoef |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Crops
Agricultural Quality Control Traceability DNA Plant bread Oligonucleotides Raw material Biochemistry Analytical Chemistry Crop pcr Labelling wheat fragrance Multiplex Food science gene Ligation Triticum Paper in Forefront basmati rice business.industry food and beverages Oryza DNA adulteration Food Analysis Authenticity Biotechnology Detection rice oryza-sativa Plant species Food processing business Rikilt B&T Novel Foods en Agroketens Oligonucleotide Probes Rikilt B&T Toxicologie en Effectanalyse Multiplex Polymerase Chain Reaction |
| Zdroj: | Analytical and Bioanalytical Chemistry Analytical and Bioanalytical Chemistry 402 (2012) 2 Analytical and Bioanalytical Chemistry, 402(2), 693-701 |
| ISSN: | 1618-2650 1618-2642 |
| Popis: | The authenticity of food is of increasing importance for producers, retailers and consumers. All groups benefit from the correct labelling of the contents of food products. Producers and retailers want to guarantee the origin of their products and check for adulteration with cheaper or inferior ingredients. Consumers are also more demanding about the origin of their food for various socioeconomic reasons. In contrast to this increasing demand, correct labelling has become much more complex because of global transportation networks of raw materials and processed food products. Within the European integrated research project ‘Tracing the origin of food’ (TRACE), a DNA-based multiplex detection tool was developed—the padlock probe ligation and microarray detection (PPLMD) tool. In this paper, this method is extended to a 15-plex traceability tool with a focus on products of commercial importance such as the emmer wheat Farro della Garfagnana (FdG) and Basmati rice. The specificity of 14 plant-related padlock probes was determined and initially validated in mixtures comprising seven or nine plant species/varieties. One nucleotide difference in target sequence was sufficient for the distinction between the presence or absence of a specific target. At least 5% FdG or Basmati rice was detected in mixtures with cheaper bread wheat or non-fragrant rice, respectively. The results suggested that even lower levels of (un-)intentional adulteration could be detected. PPLMD has been shown to be a useful tool for the detection of fraudulent/intentional admixtures in premium foods and is ready for the monitoring of correct labelling of premium foods worldwide. |
| Databáze: | OpenAIRE |
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