Tumor-derived mural-like cells coordinate with endothelial cells: role of YKL-40 in mural cell-mediated angiogenesis
| Autor: | Nipaporn Ngernyuang, Rong Shao, Wei Yan, Brooke Bentley, Ralph Francescone |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Cell Membrane Permeability Podosome Angiogenesis Mice SCID Muscle Smooth Vascular Mice VE-cadherin 0302 clinical medicine Lectins Neoplasms Cells Cultured beta Catenin 0303 health sciences Neovascularization Pathologic mural cells Cadherins VEGF Immunohistochemistry Cell biology Platelet Endothelial Cell Adhesion Molecule-1 Vascular endothelial growth factor B Endothelial stem cell Vascular endothelial growth factor A vessel permeability 030220 oncology & carcinogenesis RNA Interference musculoskeletal diseases YKL-40 Blotting Western Myocytes Smooth Muscle Transplantation Heterologous Biology Vascular endothelial growth inhibitor Article Mural cell vessel stability 03 medical and health sciences Vasculogenesis Adipokines Cell Adhesion Genetics Animals Humans Chitinase-3-Like Protein 1 Molecular Biology N-cadherin 030304 developmental biology Endothelial Cells Muscle Smooth tumor angiogenesis Actins Coculture Techniques Pericytes |
| Zdroj: | Oncogene |
| ISSN: | 1476-5594 0950-9232 |
| Popis: | Tumor neo-vasculature is characterized by spatial coordination of endothelial cells with mural cells, which delivers oxygen and nutrients. Here, we explored a key role of the secreted glycoprotein YKL-40, a mesenchymal marker, in the interaction between endothelial cells and mesenchymal mural-like cells for tumor angiogenesis. Xenotransplantation of tumor-derived mural-like cells (GSDCs) expressing YKL-40 in mice developed extensive and stable blood vessels covered with more GSDCs than those in YKL-40 gene knockdown tumors. YKL-40 expressed by GSDCs was associated with increased interaction of neural cadherin/β-catenin/smooth muscle alpha actin; thus, mediating cell-cell adhesion and permeability. YKL-40 also induced the interaction of vascular endothelial cadherin/β-catenin/actin in endothelial cells (HMVECs). In cell co-culture systems, YKL-40 enhanced both GSDC and HMVEC contacts, restricted vascular leakage, and stabilized vascular networks. Collectively, the data inform new mechanistic insights into the cooperation of mural cells with endothelial cells induced by YKL-40 during tumor angiogenesis, and also enhance our understanding of YKL-40 in both mural and endothelial cell biology. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|