eNOS genotype is without effect on circulating nitrite/nitrate level in healthy male population
| Autor: | Juliana A. Uzuelli, Jose E. Tanus-Santos, Sabrina Nagassaki, Debora C. Souza-Costa, Aline S. Marroni, Ingrid F. Metzger |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Genotype Nitric Oxide Synthase Type III Population Endogeny Biology Nitric Oxide White People Nitric oxide chemistry.chemical_compound Sex Factors Reference Values Enos Internal medicine medicine Humans Nitrite Nitrites NOx Nitrates Polymorphism Genetic Cholesterol Hematology Middle Aged biology.organism_classification Nitric oxide synthase Endocrinology chemistry Biochemistry Luminescent Measurements biology.protein Nitric Oxide Synthase Brazil |
| Zdroj: | Thrombosis Research. 115:375-379 |
| ISSN: | 0049-3848 |
| DOI: | 10.1016/j.thromres.2004.09.003 |
| Popis: | Introduction Nitric oxide (NO) plays an important role in the regulation of the cardiovascular system. It is produced by endothelial nitric oxide synthase (eNOS), which exhibits genetic polymorphisms. Although the clinically relevant polymorphism T+IhI-786C reduces eNOS-promoter activity, it is not clear whether circulating nitrite/nitrate (NOx) are affected by this polymorphism. Materials and Methods We addressed this issue by studying a homogeneous group of 200 healthy subjects (males, Caucasians, nonsmokers, 18+IBM-56 years of age, and not taking any medication). Genotypes were determined by restriction fragment length polymorphism and circulating NOx were determined by chemiluminescence. Results We found nonsignificant effects of the T+IhI-786C polymorphism on circulating NOx (mean+ALE-S.D.=52.2+ALE-21.4, 49.0+ALE-17.8, and 45.9+ALE-16.8 +A7w-mol/L for genotypes +IBw-TT,+IB0 +IBw-TC,+IB0 and +IBw-CC,+IB0 respectively) and on total plasma cholesterol concentrations (both P>.05). No correlation was found between circulating NOx and total plasma cholesterol concentrations (P>.05). Conclusions Our study provides strong evidence that the T+IhI-786C polymorphism does not affect plasma NOx concentrations, which are believed to reflect endogenous production of NO. Therefore, our results suggest that this polymorphism does not affect endogenous NO production. |
| Databáze: | OpenAIRE |
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