Multiple injections of human umbilical cord-derived mesenchymal stromal cells through the tail vein improve microcirculation and the microenvironment in a rat model of radiation myelopathy
| Autor: | Jing Zhang, Hai-Xing Mai, Lei Wang, Ke Zheng, Jia-Ning Wang, Zhi-Fang Li, Xiu-Bin Xiao, Wei-Jing Zhang, Hua You, Li Wei, Yuan Liu, Zai-Liang Yang, Wan-Liang Sun, Yan-Qing Wang, Feng Liang |
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| Rok vydání: | 2014 |
| Předmět: |
Tail
medicine.medical_specialty Pathology Radiation myelopathy Mesenchymal Stem Cell Transplantation Umbilical cord Spinal cord blood flow General Biochemistry Genetics and Molecular Biology Spinal Cord Diseases Microcirculation Umbilical Cord Veins White matter Endothelial cell Neurotrophic factors Glial cell line-derived neurotrophic factor Paralysis Medicine Animals Medicine(all) biology Paracrine system business.industry Biochemistry Genetics and Molecular Biology(all) Research Mesenchymal stem cell Human umbilical cord-derived mesenchymal stromal cells Cell Differentiation Mesenchymal Stem Cells General Medicine Spinal cord Surgery Rats Disease Models Animal Radiation Injuries Experimental medicine.anatomical_structure Spinal Cord biology.protein medicine.symptom business |
| Zdroj: | Journal of Translational Medicine |
| ISSN: | 1479-5876 |
| Popis: | Background At present, no effective clinical treatment is available for the late effects of radiation myelopathy. The aim of the present study was to assess the therapeutic effects of human umbilical cord-derived mesenchymal stromal cells (UC-MSCs) in a rat model of radiation myelopathy. Methods An irradiated cervical spinal cord rat model was generated. UC-MSCs were injected through the tail vein at 90, 97, 104 and 111 days post-irradiation. Behavioral tests were performed using the forelimb paralysis scoring system, and histological damage was examined using Nissl staining. The microcirculation in the spinal cord was assessed using von Willebrand factor (vWF) immunohistochemical analysis and laser-Doppler flowmetry. The microenvironment in the spinal cord was determined by measuring the pro-inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the serum and the anti-inflammatory cytokines brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) in the spinal cord. Results Multiple injections of UC-MSCs through the tail veil decreased the forelimb paralysis, decreased spinal cord histological damage, increased the number of neurons in the anterior horn of the spinal cord, increased the endothelial cell density and the microvessel density in the white matter and gray matter of the spinal cord, increased the relative magnitude of spinal cord blood flow, down-regulated pro-inflammatory cytokine expression in the serum, and increased anti-inflammatory cytokine expression in the spinal cord. Conclusion Multiple injections of UC-MSCs via the tail vein in a rat model of radiation myelopathy significantly improved the microcirculation and microenvironment through therapeutic paracrine effects. |
| Databáze: | OpenAIRE |
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