Cocaine induces differential circular RNA expression in striatum
| Autor: | Wei Guo, Yinglan Zhao, Bin Liu, Qian Bu, Jueying Kong, Hailei Long, Li Luo, Xiaobo Cen, Jingwei Tian, Hui Gu, Hongbo Wang, Xue Shao |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Down-Regulation Self Administration LIMK1 Biology Molecular neuroscience Article lcsh:RC321-571 03 medical and health sciences Cellular and Molecular Neuroscience Mice 0302 clinical medicine Cocaine Dopamine Uptake Inhibitors Circular RNA Transcription (biology) Epigenetics and behaviour microRNA Animals Gene lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry Biological Psychiatry Microarray analysis techniques RNA RNA Circular Corpus Striatum Cell biology Up-Regulation Psychiatry and Mental health 030104 developmental biology Gene Expression Regulation Synaptic plasticity 030217 neurology & neurosurgery |
| Zdroj: | Translational Psychiatry Translational Psychiatry, Vol 9, Iss 1, Pp 1-11 (2019) |
| ISSN: | 2158-3188 |
| Popis: | Circular RNA (circRNA), a novel type of endogenous non-coding RNA, plays natural miRNA sponge effect that represses the activities of corresponding miRNAs through binding with them, thus modulating transcriptional expression of genes. Recent studies indicate that circRNAs are significantly enriched in the brain and some of them are derived from synaptic protein-coding genes. In addition, miRNAs are involved in synaptic plasticity, memory formation, and cocaine addiction. However, the role of circRNAs in cocaine reward is unclear. This study aimed to investigate the expression profile of striatal circRNAs in the mice after cocaine self-administration. By using circRNA microarray analysis, we observed that 90 striatal circRNAs were differentially expressed in cocaine self-administering mice, of which 18 circRNAs were up-regulated and 72 down-regulated. Six circRNAs were selected randomly for validation by using quantitative reverse transcription-PCR, and their expression levels showed consistency with microarray analysis. We backward predicted the circRNAs and their binding sites of miRNAs associated with neuroplasticity. In functional validation test, mmu_circRNA_002381 may modulate the transcription of certain genes associated with neuroplasticity, such as limk1 and bdnf. Taken together, circRNAs may participate in cocaine behavioral effect via interacting with miRNAs. Our findings reveal a potential role of circRNAs in cocaine effect. |
| Databáze: | OpenAIRE |
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