Characterization of Eleusine indica with gene mutation or amplification in EPSPS to glyphosate
| Autor: | Jingchao Chen, Shouhui Wei, Hongjuan Huang, Cuilan Jiang, Chaoxian Zhang, Dandan Zhao, Huimin Wang, Zhaofeng Huang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Health Toxicology and Mutagenesis Population Glycine Eleusine indica Gene mutation 01 natural sciences Eleusine chemistry.chemical_compound Gene Expression Regulation Plant Botany education Gene Glutathione Transferase Plant Proteins Genetics education.field_of_study biology Herbicides Gene Amplification 04 agricultural and veterinary sciences General Medicine biology.organism_classification Enzyme assay chemistry Susceptible individual Glyphosate Mutation 040103 agronomy & agriculture biology.protein 0401 agriculture forestry and fisheries 3-Phosphoshikimate 1-Carboxyvinyltransferase Weed Agronomy and Crop Science 010606 plant biology & botany Herbicide Resistance |
| Zdroj: | Pesticide biochemistry and physiology. 143 |
| ISSN: | 1095-9939 |
| Popis: | The evolution of weed-resistant species threatens the sustainable use of glyphosate, which is the most important herbicide widely used in agriculture worldwide. Moreover, the high glyphosate resistance (>180-fold based on LD50) of Eleusine indica found in Malaysia, which carries a double mutation in its 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), made the control of this species more difficult. By contrast, the same species carrying the same double mutation in EPSPS (T102I+P106S) but found in China only shows a resistance level of not more than 14-fold based on GR50. The resistance level of this population is four times higher than that of the population carrying a single mutation (P106L). Although the members of this population survive under a high glyphosate dosage of 10,080gaeha-1, their growth was significantly inhibited by glyphosate under the recommend dose (840gaeha-1), where in the fresh weight was 85.4% of the control. EPSPS expression, relative copy number, and EPSPS activity in this population were similar to those of the susceptible population. In addition, the expression of two glutathione transferase (GST) genes (GST-U8 and GST-23) and the enzyme activity of the GST in this population did not significantly differ from those of the susceptible population. This finding is important in elucidating the resistance of the naturally evolved glyphosate-resistant (GR) weed species carrying a double mutation in EPSPS to glyphosate. |
| Databáze: | OpenAIRE |
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