Aphid growth and reproduction on plants with altered sterol profiles: Novel insights using Arabidopsis mutant and overexpression lines
| Autor: | Keyan Zhu-Salzman, Robert J. Grebenok, Ivy W Chen, Hubert Schaller, Spencer T. Behmer |
|---|---|
| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Food Chain Physiology Campesterol Arabidopsis 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Botany polycyclic compounds Arabidopsis thaliana Animals [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Aphid Stigmasterol biology Phytosterol fungi food and beverages Phytosterols biochemical phenomena metabolism and nutrition biology.organism_classification Plants Genetically Modified Sitosterols Sterol 010602 entomology 030104 developmental biology Cholesterol chemistry Insect Science Aphids lipids (amino acids peptides and proteins) Myzus persicae |
| Zdroj: | Journal of Insect Physiology Journal of Insect Physiology, Elsevier, 2020, 123, pp.104054. ⟨10.1016/j.jinsphys.2020.104054⟩ |
| ISSN: | 0022-1910 |
| Popis: | Sterols are essential membrane components and are critical for many physiological processes in all eukaryotes. Insects and other arthropods are sterol auxotrophs that typically rely on a dietary source of sterols. Herbivorous insects generally obtain sterols from plants and then metabolize them into cholesterol, the dominant sterol in most insects. However, there is significant variation in phytosterol structure, and not all phytosterols are equally suitable for insects. In the current study, we used seven Arabidopsis thaliana lines that display altered sterol profiles due to mutations in the sterol biosynthetic pathway or to overexpression of key enzymes of the pathway, and investigated how plant sterol profiles affected green peach aphid (Myzus persicae) growth and reproduction. We also characterized the sterol profile of aphids reared on these Arabidopsis genotypes. Aphids on two mutant lines (14R/fk and ste1-1) that accumulated biosynthetic sterol intermediates (Δ8,14-sterols, and Δ7-sterols, respectively) all showed significantly reduced growth and reproduction. Aphids on SMT2COSUP plants (which have decreased β-sitosterol but increased campesterol) also displayed significantly reduced growth and reproduction. However, aphids on SMT2OE plants (which have increased β-sitosterol but decreased campesterol) performed similarly to aphids on wild-type plants. Finally, Arabidopsis plants that had an overproduction of sterols (CD-HMGROE) or decreased sterol esters (psat1-2) had no impact on aphid performance. Two noteworthy results come from the aphid sterol profile study. First, β-sitosterol, cholesterol and stigmasterol were recovered in all aphids. Second, we did not detect Δ8,14-sterols in aphids reared on 14R/fk plants. We discuss the implications of our findings, including how aphid sterol content does not appear to reflect plant leaf sterol profiles. We also discuss the potential of modifying plant sterol profiles to control insect herbivore pests, including aphids. |
| Databáze: | OpenAIRE |
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