Two novel direct SPIO labels and in vivo MRI detection of labeled cells after acute myocardial infarct
| Autor: | Elisa Lappi-Blanco, Petri Lehenkari, Tatu Juvonen, Riikka M Korpi, Ronald Borra, Fredrik Yannopoulos, Vesa Anttila, Roberto Blanco Sequieros, Siri Lehtonen, Kirsi Alestalo, Jarkko Korpi, Eveliina Lammentausta, Timo Ruuska |
|---|---|
| Přispěvatelé: | Department of Diagnostics and Therapeutics, Korva-, nenä- ja kurkkutautien klinikka, HUS Head and Neck Center, Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
magnetic resonance imaging (MRI) Cell superparamagnetic iron oxide (SPIO) particles 030204 cardiovascular system & hematology bone marrow mononuclear cells (BMMNCs) 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine In vivo Internal medicine medicine Journal Article Myocardial infarction Magnetic resonance imaging (MRI) ta3126 business.industry Research acute myocardial infarct (AMI) 217 Medical engineering General Medicine Hypoxia (medical) medicine.disease 3. Good health Transplantation medicine.anatomical_structure Cardiology Radiology medicine.symptom business transplantation |
| Zdroj: | Acta Radiologica Open Acta radiologica open, 6(8). SAGE Publications Inc. |
| ISSN: | 2058-4601 |
| Popis: | Background Acute myocardial infarction (AMI) is a leading cause of morbidity and mortality worldwide. Cellular decay due hypoxia requires rapid and validated methods for possible therapeutic cell transplantation. Purpose To develop direct and rapid superparamagnetic iron oxide (SPIO) cell label for a large-animal model and to assess in vivo cell targeting by magnetic resonance imaging (MRI) in an experimental AMI model. Material and Methods Bone marrow mononuclear cells (BMMNCs) were labeled with SPIO particles using two novel direct labeling methods (rotating incubation method and electroporation). Labeling, iron incorporation in cells and label distribution, cellular viability, and proliferation were validated in vitro. An AMI porcine model was used to evaluate the direct labeling method (rotating incubation method) by examining targeting of labeled BMMNCs using MRI and histology. Results Labeling (1 h) did not alter either cellular differentiation potential or viability of cells in vitro. Cellular relaxation values at 9.4 T correlated with label concentration and MRI at 1.5 T showing 89 ± 4% signal reduction compared with non-labeled cells in vitro. In vivo, a high spatial correlation between MRI and histology was observed. The extent of macroscopic pathological myocardial changes (hemorrhage) correlated with altered function detected on MRI. Conclusion We demonstrated two novel direct SPIO labeling methods and demonstrated the feasibility of clinical MRI for monitoring targeting of the labeled cells in animal models of AMI. |
| Databáze: | OpenAIRE |
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