Cloning and characterization of the pseudonajatoxin b precursor
| Autor: | NanLing GONG, Arunmozhiarasi ARMUGAM, Peter MIRTSCHIN, Kandiah JEYASEELAN |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Elapid Venoms
Base Sequence Sequence Homology Amino Acid Molecular Sequence Data Neurotoxins Restriction Mapping Australia Cell Biology Regulatory Sequences Nucleic Acid Polymerase Chain Reaction Biochemistry Recombinant Proteins Genes Liver Animals Amino Acid Sequence Elapidae Cloning Molecular Protein Precursors Promoter Regions Genetic Sequence Alignment Molecular Biology Phylogeny Research Article |
| Zdroj: | Biochemical Journal. 358:647-656 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3580647 |
| Popis: | An Australian common brown snake, Pseudonaja textilis, is known to contain highly lethal neurotoxins. Among them, a long-chain alpha-neurotoxin, pseudonajatoxin b, has been identified. In this report, while presenting evidence for the presence of at least four such long-chain alpha-neurotoxins in the venom of P. textilis, we describe the characteristics of both the mRNA and the gene responsible for the synthesis of these neurotoxins. A precursor toxin synthesized from the gene has been identified as being capable of producing the isoforms possibly by post-translational modifications at its C-terminal end. Recombinant toxins corresponding to the precursor and its product have been found to possess similar binding affinities for muscular acetylcholine receptors (IC(50)=3x10(-8) M) and a lethality, LD(50), of 0.15 microg/g in mice. |
| Databáze: | OpenAIRE |
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