Bik reduces hyperplastic cells by increasing Bak and activating DAPk1 to juxtapose ER and mitochondria
| Autor: | Neal Lacey, Ivan Leyva-Baca, Hitendra S. Chand, Augustine M.K. Choi, Marc G. Wathelet, Yohannes A. Mebratu, Yohannes Tesfaigzi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
General Physics and Astronomy Apoptosis Mitochondrion Endoplasmic Reticulum Mice 0302 clinical medicine Smoke lcsh:Science Cells Cultured Mice Knockout Multidisciplinary biology Kinase Signal transducing adaptor protein Tobacco Products Mitochondria 3. Good health Cell biology bcl-2 Homologous Antagonist-Killer Protein Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis biological phenomena cell phenomena and immunity Bcl-2 Homologous Antagonist-Killer Protein Calmodulin Science chemistry.chemical_element Respiratory Mucosa Calcium Article General Biochemistry Genetics and Molecular Biology Mitochondrial Proteins 03 medical and health sciences Animals Humans Adaptor Proteins Signal Transducing Hyperplasia Endoplasmic reticulum Epithelial Cells General Chemistry Allergens Death-Associated Protein Kinases 030104 developmental biology chemistry biology.protein lcsh:Q Apoptosis Regulatory Proteins Peptides |
| Zdroj: | Nature Communications Nature Communications, Vol 8, Iss 1, Pp 1-14 (2017) |
| ISSN: | 2041-1723 |
| Popis: | Bik reduces hyperplastic epithelial cells by releasing calcium from endoplasmic reticulum stores and causing apoptosis, but the detailed mechanisms are not known. Here we report that Bik dissociates the Bak/Bcl-2 complex to enrich for ER-associated Bak and interacts with the kinase domain of DAPk1 to form Bik–DAPk1–ERK1/2–Bak complex. Bik also disrupts the Bcl2–IP3R interaction to cause ER Ca2+ release. The ER-associated Bak interacts with the kinase and calmodulin domains of DAPk1 to increase the contact sites of ER and mitochondria, and facilitate ER Ca2+ uptake by mitochondria. Although the Bik BH3 helix was sufficient to enrich for ER-Bak and elicit ER Ca2+ release, Bik-induced mitochondrial Ca2+ uptake is blocked with reduced Bak levels. Further, the Bik-derived peptide reduces allergen- and cigarette smoke-induced mucous cell hyperplasia in mice and in differentiated primary human airway epithelial cultures. Therefore, Bik peptides may have therapeutic potential in airway diseases associated with chronic mucous hypersecretion. Bcl-2 interacting killer (Bik) decreases airway epithelial hyperplasia via apoptosis mediated by calcium release from the endoplasmic reticulum (ER), but the mechanism is unclear. Here the authors show that Bik promotes Bak enrichment at the ER to tether mitochondria for efficient calcium transfer. |
| Databáze: | OpenAIRE |
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