Dengue Infection in Children in Fortaleza, Brazil: A 3-Year School-Based Prospective Cohort Study
| Autor: | Adrienne Guignard, Fernanda Montenegro de Carvalho Araújo, Ana Claudia Machado Duarte, Catherine Minguet, Ivo Castelo Branco Coelho, Jeová Keny Baima Colares, Beatriz S. Borges, François Haguinet, Waleska Dias Schwarcz, Zirlane Castelo Branco Coêlho |
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| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Adolescent 030231 tropical medicine Dengue virus Antibodies Viral medicine.disease_cause Severity of Illness Index Asymptomatic Dengue fever Dengue 03 medical and health sciences 0302 clinical medicine Plaque reduction neutralization test Neutralization Tests Seroepidemiologic Studies Virology Internal medicine Humans Medicine Seroprevalence Prospective Studies Child Prospective cohort study Asymptomatic Infections Disease Notification business.industry Incidence Incidence (epidemiology) Articles Dengue Virus medicine.disease Infectious Diseases Child Preschool Immunoglobulin G Epidemiological Monitoring Female Parasitology medicine.symptom business Brazil |
| Zdroj: | The American Journal of Tropical Medicine and Hygiene |
| ISSN: | 1476-1645 0002-9637 |
| Popis: | Dengue is endemic in Brazil. The dengue surveillance system’s reliance on passive reporting may underestimate disease incidence and cannot detect asymptomatic/pauci-symptomatic cases. In this 3-year prospective cohort study (NCT01391819) in 5- to 13-year-old children from nine schools in Fortaleza (N = 2,117), we assessed dengue virus (DENV) infection seroprevalence by IgG indirect ELISA at yearly visits and disease incidence through active and enhanced passive surveillance. Real-time quantitative polymerase chain reaction (RT-qPCR) and DENV IgM/IgG capture ELISA were used for diagnosis. We further characterized confirmed and probable cases with a plaque reduction neutralization test. At enrollment, 54.1% (95% CI: 46.6, 61.4) of children were DENV IgG positive. The annual incidence of laboratory-confirmed symptomatic dengue cases was 11.0 (95% CI: 7.3, 14.7), 18.1 (10.4, 25.7), and 10.2 (0.7, 19.7), and of laboratory-confirmed or probable dengue cases with neutralizing antibody profile evocative of dengue exposure was 13.2 (6.6, 19.9), 18.7 (5.3, 32.2), and 8.4 (2.4, 19.2) per 1,000 child-years in 2012, 2013, and 2014, respectively. By RT-qPCR, we identified 14 DENV-4 cases in 2012–2013 and seven DENV-1 cases in 2014. During the course of the study, 32.8% of dengue-naive children experienced a primary infection. Primary inapparent dengue infection was detected in 20.3% (95% CI: 13.6, 29.1) of dengue-naive children in 2012, 8.7% (6.9, 10.9) in 2013, and 5.1% (4.4, 6.0) in 2014. Our results confirmed the high dengue endemicity in Fortaleza, with active and enhanced passive surveillance detecting three to five times more cases than the National System of Disease Notification. |
| Databáze: | OpenAIRE |
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