In vitro study on antioxidant, antibacterial and DNA interaction activities of extracts from Arbutus andrachne L
| Autor: | Ömer Ertürk, Melek Gul, Ceren Baskan, Güler İnci Tanrıkulu, Dudu Duygu Kiliç, Belgin Siriken |
|---|---|
| Přispěvatelé: | OMÜ |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
040101 forestry
chemistry.chemical_classification Arbutus andrachne L. antioxidant antimicrobial DNA interaction Metal chelating activity Antioxidant Fen biology Arbutus andrachne Chemistry DPPH medicine.medical_treatment Science Flavonoid Bacillus cereus 04 agricultural and veterinary sciences General Medicine biology.organism_classification Antimicrobial chemistry.chemical_compound medicine 0401 agriculture forestry and fisheries Food science Orman Mühendisliği Bacteria |
| Zdroj: | Volume: 7, Issue: 3 293-300 Eurasian Journal of Forest Science |
| ISSN: | 2147-7493 |
| Popis: | Traditionally, the fruits and leaves of Arbutus andrachne are well known and used in for applications in antimicrobial, antioxidant and anticancer activity. This study was aimed to determine the in vitro antibacterial, antioxidant and DNA interaction activities of the different extracts of Arbutus andrachne.Arbutus andrachne was collected from Amasya Province during May 2018. Dried leaves powder were mixed with of suitable solution. Then, the mixtures were extracted with the soxhlet apparatus for 4 hours. The antibacterial effects of extracts were researched on pathogens, namely Staphylococcus aureus ATCC 25923, Staphylococcus aureus ATCC 25953, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa ATCC 9027, Bacillus cereus ATCC 7064, Bacillus subtilis ATCC 6633, and Salmonella enteritidis ATCC 13076 using disc diffusion methods. Gentamicin and cefotaxime were used as control for bacteria. To try to understand each plant extracts effect on the antioxidant methods, we studied the different methods as named radical scavenging activity (DPPH), metal chelating activity, ferric reducing antioxidant power assays. In the plant extracts, total phenolic and total flavonoid contents were determined by spectrophotometric methods due to investigating the effect of secondary metabolites on antioxidant activity. To explore the beneficial effect of the extracts on hydroxyl radical-mediated DNA damage plasmid DNA pUC18 was used.As a result, Arbutus andrachne extracts had antibacterial effect when they compared with control group antibiotics. According to disc diffusion methods, the highest antibacterial effect was identified in ethanol, chloroform and hexane extract, respectively. Ethanol extracts of Arbutus andrachne showed the best antioxidant activity. Moreover, extracts of Arbutus andrachne had repair effects on plasmid DNA in H2O2 condition. The investigated Arbutus andrachne extract showed significant bioactivities. These extracts have been a promising candidate for the preparation of new natural products. However, future studies should be carried out to verify such actions in different matrices. |
| Databáze: | OpenAIRE |
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