| Autor: |
Miho Akutsu, Nanase Abe, Chiho Sakamoto, Yuki Kurimoto, Haruka Sugita, Makoto Tanaka, Yudai Higuchi, Kimitoshi Sakamoto, Naofumi Kamimura, Hiroyuki Kurihara, Eiji Masai, Tomonori Sonoki |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Bioresource Technology. 359:127479 |
| ISSN: |
0960-8524 |
| Popis: |
cis,cis-Muconate (ccMA) is a promising platform for use in synthesizing various polymers. A glucose-free ccMA production using Pseudomonas sp. NGC7 from hardwood lignin-derived aromatic compounds was previously reported. In that system, syringyl nucleus compounds were essential for growth. Here, it is shown that NGC7 is available for glucose-free ccMA production even from a mixture of lignin-derived aromatics that does not contain syringyl nucleus compounds. By introducing a gene set for the protocatechuate (PCA)-shunt consisting of PCA 3,4-dioxygenase and PCA decarboxylase into an NGC7-derived strain deficient in PCA 3,4-dioxygenase and ccMA cycloisomerase, it was succeeded in constructing a ccMA-producing strain that grows on a lignin-derived aromatics mixture containing no syringyl nucleus compounds. Finally, it is demonstrated that the engineered strain produced ccMA from sugar cane bagasse alkaline extract in 18.7 mol%. NGC7 is thus shown to be a promising microbial chassis for biochemicals production from lignin-derived aromatics. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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