Detection of Clostridium difficile in Stool Samples From Patients in the Early Period After Liver Transplantation
Autor: | Leszek Paczek, A. Rokosz, Wojciech Rowinski, Dariusz Kawecki, L. Adadynski, A. Szymanowska, Piotr Małkowski, Anna Sawicka-Grzelak, Dariusz Wasiak, Magdalena Durlik, Ewa Swoboda-Kopeć, Andrzej Chmura, Mirosław Luczak, Marta Wróblewska, Marek Pacholczyk, B. Lagiewska |
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Rok vydání: | 2007 |
Předmět: |
Adult
Male medicine.medical_specialty medicine.medical_treatment Bacterial Toxins Stool specimen Liver transplantation medicine.disease_cause Gastroenterology Enterotoxins Feces Postoperative Complications Internal medicine medicine Humans Clostridiaceae Postoperative Period Enterocolitis Pseudomembranous Aged Retrospective Studies Cross Infection Transplantation biology medicine.diagnostic_test Clostridioides difficile Toxin business.industry Middle Aged Clostridium difficile biology.organism_classification Liver Transplantation Diarrhea Immunoassay Immunology Female Surgery medicine.symptom business |
Zdroj: | Transplantation Proceedings. 39:2812-2815 |
ISSN: | 0041-1345 |
Popis: | Objective We examined the frequency of detection of Clostridium difficile (CD) toxins compared with the recovery of C. difficile in stool specimen cultures among orthotopic liver transplant (OLT) patients with nosocomial diarrhea in the early period. Materials and Methods The study included stool samples obtained during the first 30 days after OLT in adults who were suspected of CD-associated diseases. The identification of cultured CD strains was performed by standard microbiological methods. The presence of CD toxins was assayed using a commercial immunoassay. Results All patients were followed prospectively for CD infections from the date of OLT for the first 4 weeks after surgery. Among 54 samples, 16.7% were culture-positive for CD. CD toxins were tested on 54 samples, yielding 63% toxin-positive samples and 30% toxin- and culture-negative results. In the first week after OLT, samples from 19 patients were subjected to CD investigation. Among 19 samples positive for toxin, 52.6% of all samples were culture-negative. We analyzed 35 samples from the second to the fourth week after OLT in 31 recipients. Among 35 samples, 68.6% and 25.7% were positive for CD toxin and for culture, while 20% of samples were negative for toxin and culture. Conclusion In our study, 63% of samples were toxin-positive with 16.7% yielding growth of CD and 30% being negative for toxins and cultures. |
Databáze: | OpenAIRE |
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