The Presence of Anti-HLA Antibodies before and after Allogeneic Hematopoietic Stem Cells Transplantation from HLA-Mismatched Unrelated Donors
| Autor: | Miroslaw Markiewicz, Anna Koclęga, Slawomira Kyrcz-Krzemien, Patrycja Zielinska, Malgorzata Sobczyk Kruszelnicka, Mizia Sylwia, Monika Dzierzak-Mietla, Alicja Dobrowolska, Andrzej Lange, Urszula Siekiera |
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| Rok vydání: | 2012 |
| Předmět: |
Article Subject
biology lcsh:RC633-647.5 business.industry Immunogenicity medicine.medical_treatment Immunology Panel reactive antibody lcsh:Diseases of the blood and blood-forming organs Cell Biology Hematology Human leukocyte antigen Hematopoietic stem cell transplantation Transplantation Haematopoiesis surgical procedures operative Clinical Study biology.protein Medicine Antibody Stem cell business |
| Zdroj: | Bone Marrow Research Bone Marrow Research, Vol 2012 (2012) |
| ISSN: | 2090-3006 2090-2999 |
| DOI: | 10.1155/2012/539825 |
| Popis: | Although anti-human leukocyte antigen antibodies (anti-HLA Abs) are important factors responsible for graft rejection in solid organ transplantation and play a role in post-transfusion complications, their role in allogeneic hematopoietic stem cell transplantation (allo-HSCT) has not been finally defined. Enormous polymorphism of HLA-genes, their immunogenicity and heterogeneity of antibodies, as well as the growing number of allo-HSCTs from partially HLA-mismatched donors, increase the probability that anti-HLA antibodies could be important factors responsible for the treatment outcomes. We have examined the incidence of anti-HLA antibodies in a group of 30 allo-HSCT recipients from HLA-mismatched unrelated donors. Anti-HLA Abs were identified in sera collected before and after allo-HSCT. We have used automated DynaChip assay utilizing microchips bearing purified class I and II HLA antigens for detection of anti-HLA Abs. We have detected anit-HLA antibodies against HLA-A, B, C, DR, DQ and DP, but no donor or recipient-specific anti-HLA Abs were detected in the studied group. The preliminary results indicate that anti-HLA antibodies are present before and after allo-HSCT in HLA-mismatched recipients. |
| Databáze: | OpenAIRE |
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