Tissue-engineered heart valves develop native-like collagen fiber architecture
| Autor: | Fpt Frank Baaijens, Maj Martijn Cox, Cvc Carlijn Bouten, Njb Niels Driessen, Jeroen Kortsmit |
|---|---|
| Přispěvatelé: | Soft Tissue Biomech. & Tissue Eng. |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Tissue engineered
Materials science Microscopy Confocal Sheep Tissue Engineering Fibrillar Collagens Biomedical Engineering Bioengineering Biochemistry Heart Valves Models Biological Biomaterials Heart valve tissue engineering Confocal imaging Tissue engineering Collagen fiber Indentation Heart Valve Prosthesis Animals Fiber architecture Fiber Biomedical engineering Mechanical Phenomena |
| Zdroj: | Tissue engineering. Part A, 16(5), 1527-1537. Mary Ann Liebert Inc. |
| ISSN: | 1937-3341 |
| Popis: | Creating autologous tissues with on-demand and native-like biomechanical properties is the ultimate challenge in functional heart valve tissue engineering. A promising approach toward this goal is to induce development of native-like tissue structure in vitro by mimicking the diastolic loading phase in a bioreactor. Heart valves cultured with this approach showed in vitro sufficient strength to withstand systemic pressures. This study aims to link global functioning of these valves to the development of a native-like fiber architecture induced by in vitro diastolic loading. It is hypothesized that increased loading magnitude during culture will lead to increased collagen fiber alignment. To test this hypothesis, 10 tissue-engineered heart valves were subjected to different loading protocols in vitro. Local fiber distribution and mechanics were determined in an inverse numerical-experimental approach, combining indentation tests with confocal imaging. Indentation tests on native ovine heart valves were used as a comparison. Although the effect of loading magnitude was small within the tested range, results indicated that the local fiber architecture indeed developed toward native structural properties for all loading protocols. However, apparent fiber mechanics were much stiffer compared with native. This confirms that in vitro mechanical conditioning induces development of a native-like tissue architecture, which underlines its importance for functional heart valve tissue engineering. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|