Metataxonomic profiling of bacterial communities and their predictive functional profiles in traditionally preserved meat products of Sikkim state in India
| Autor: | Jyoti Prakash Tamang, Meera Ongmu Bhutia, H. Nakibapher Jones Shangpliang, Namrata Thapa |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
030309 nutrition & dietetics
Firmicutes Lactococcus India Chryseobacterium 03 medical and health sciences 0404 agricultural biotechnology Animals Food science Psychrobacter 0303 health sciences Bacteria biology food and beverages Bacteroidetes 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Bdellovibrio Meat Products Sikkim Bdellovibrio bacteriovorus Cattle Proteobacteria Food Science |
| Zdroj: | Food Research International. 140:110002 |
| ISSN: | 0963-9969 |
| DOI: | 10.1016/j.foodres.2020.110002 |
| Popis: | Traditionally preserved meat products are common food items in Sikkim state of India. We studied the high-throughput sequencing of four traditionally preserved meat products viz. beef kargyong, pork kargyong, yak satchu and khyopeh to profile the bacterial communities and also inferred their predictive functional profiles. Overall abundant OTUs in samples showed that Firmicutes was the abundant phylum followed by Proteobacteria and Bacteroidetes. Abundant species detected in each product were Psychrobacter pulmonis in beef kargyong, Lactobacillus sakei in pork kargyong, Bdellovibrio bacteriovorus and Ignatzschinera sp. in yak satchu and Lactobacillus sakei and Enterococcus sp. in khyopeh. Several genera unique to each product, based on analysis of shared OTUs contents, were observed among the samples except in khyopeh. Goods coverage recorded to 1.0 was observed, which reflected the maximum bacterial diversity in the samples. Alpha diversity metrics showed a maximum bacterial diversity in khyopeh and lowest in pork kargyong Community dissimilarities in the products were observed by PCoA plot. A total of 133 KEGG predictive functional pathways was observed in beef kargyong, 131 in pork kargyong, 125 in yak satchu and 101 in khyopeh. Metagenome contribution of the OTUs was computed using PICTRUSt2 and visualized by BURRITO software to predict the metabolic pathways. Several predictive functional profiles were contributed by abundant OTUs represented by Enterococcus, Acinetobacter, Agrobacterium, Bdellovibrio, Chryseobacterium, Lactococcus, Leuconostoc, Psychrobacter, and Staphylococcus. |
| Databáze: | OpenAIRE |
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