Multilayered Porous Titanium-Based 3rd Generation Biomaterial Designed for Endosseous Implants
| Autor: | Olga Soritau, Alexandra Caziuc, Catalin Popa, Maximilian George Dindelegan, Vasile Bintintan, Violeta Paşcalău, Ioana Brie, G. Dindelegan, Carmen Mihaela Mihu |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
Population BMP2 02 engineering and technology engineering.material Bone morphogenetic protein lcsh:Technology Bone morphogenetic protein 2 Article Chitosan 03 medical and health sciences chemistry.chemical_compound Coating growth factors General Materials Science titanium Bovine serum albumin lcsh:Microscopy education lcsh:QC120-168.85 030304 developmental biology 0303 health sciences education.field_of_study lcsh:QH201-278.5 biology lcsh:T IGF1 biomaterial Biomaterial Microcarrier 021001 nanoscience & nanotechnology microspheres chemistry lcsh:TA1-2040 biology.protein engineering lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering lcsh:Engineering (General). Civil engineering (General) 0210 nano-technology lcsh:TK1-9971 Biomedical engineering |
| Zdroj: | Materials, Vol 14, Iss 1727, p 1727 (2021) Materials Volume 14 Issue 7 |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma14071727 |
| Popis: | This work proposes a novel complex multi-layered material consisting of porous titanium as a substrate and a complex coating consisting of a chitosan film engulfing microsphere loaded with growth factors such as BMP2 (bone morphogenic protein 2) and IGF1 (insulin-like growth factor-1). The microspheres were obtained through deposition of dual layers of calcium cross linked pectin–chitosan/pectin polyelectrolyte onto a BSA (bovine serum albumin) gel core. The multilayer was conceived to behave like a 3rd generation biomaterial, by slow delivery of viable growth factors around implants, and to assist the healing of implantation wound and the development of new vital bone. The biologic effect of the delivery of growth factors was studied in vitro, on MSC-CD1 mesenchymal stem cells, and in vivo, on CD1 mice. Proliferation and differentiation of cells were accelerated by growth factors, especially IGF1 for proliferation and BMP2 for differentiation. In vivo tests analyzed histologically and by MicroCT show a more structured tissue around BMP2 samples. The present concept will give the best clinical results if both growth factors are delivered together by a coating film that contains a double population of microcarriers. |
| Databáze: | OpenAIRE |
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