Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination
| Autor: | Estienne C. Swart, Miroslav Arambasic, Mariusz Nowacki, Michael Ignarski, Pamela Y. Sandoval, Aditi Singh |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Transposable element
Cell Survival Protozoan Proteins Biology Genome Epigenesis Genetic Histones 03 medical and health sciences 0302 clinical medicine Macronucleus Genetics Paramecium Chromatin Assembly Factor-1 Epigenetics 030304 developmental biology 0303 health sciences Reproduction Gene regulation Chromatin and Epigenetics DNA Protozoan biology.organism_classification Chromatin 570 Life sciences biology RNA Small Untranslated Paramecium tetraurelia RNA Protozoan 030217 neurology & neurosurgery |
| Zdroj: | Nucleic Acids Research Ignarski, Michael; Singh, Aditi; Swart, Estienne Carl; Arambasic, Miroslav; Sandoval, Pamela Yohanna de Lourdes; Nowacki, Mariusz (2014). Paramecium tetraurelia chromatin assembly factor-1-like protein PtCAF-1 is involved in RNA-mediated control of DNA elimination. Nucleic acids research, 42(19), pp. 11952-11964. Information Retrieval Ltd. 10.1093/nar/gku874 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gku874 |
| Popis: | Genome-wide DNA remodelling in the ciliate Paramecium is ensured by RNA-mediated trans-nuclear crosstalk between the germline and the somatic genomes during sexual development. The rearrangements include elimination of transposable elements, minisatellites and tens of thousands non-coding elements called internally eliminated sequences (IESs). The trans-nuclear genome comparison process employs a distinct class of germline small RNAs (scnRNAs) that are compared against the parental somatic genome to select the germline-specific subset of scnRNAs that subsequently target DNA elimination in the progeny genome. Only a handful of proteins involved in this process have been identified so far and the mechanism of DNA targeting is unknown. Here we describe chromatin assembly factor-1-like protein (PtCAF-1), which we show is required for the survival of sexual progeny and localizes first in the parental and later in the newly developing macronucleus. Gene silencing shows that PtCAF-1 is required for the elimination of transposable elements and a subset of IESs. PTCAF-1 depletion also impairs the selection of germline-specific scnRNAs during development. We identify specific histone modifications appearing during Paramecium development which are strongly reduced in PTCAF-1 depleted cells. Our results demonstrate the importance of PtCAF-1 for the epigenetic trans-nuclear cross-talk mechanism. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|