MiR-216a-5p alleviates chronic constriction injury-induced neuropathic pain in rats by targeting KDM3A and inactivating Wnt/β-catenin signaling pathway
| Autor: | Weining Wang, Renchao Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Jumonji Domain-Containing Histone Demethylases Pharmacology Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation microRNA Animals Medicine Wnt Signaling Pathway Neuroinflammation Gene knockdown business.industry General Neuroscience Wnt signaling pathway General Medicine Constriction Rats MicroRNAs 030104 developmental biology Mir 216a Neuropathic pain Neuralgia Signal transduction business 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Research. 170:255-264 |
| ISSN: | 0168-0102 |
| Popis: | Neuropathic pain is a devastating disease and exists tolerance to current available analgesics. MicroRNAs were reported to be involved in the regulation of neuropathic pain, but the biological role of miR-216a-5p in neuropathic pain remains unclear. In this study, we constructed a CCI rat model of neuropathic pain. Our results showed that the expression of miR-216a-5p was downregulated in CCI rats, and mechanical allodynia and thermal hyperalgesia in CCI rats were improved by miR-216a-5p overexpression, suggesting that miR-216a-5p overexpression alleviated neuropathic pain. Moreover, ELISA showed that miR-216a-5p overexpression inhibited concentration and mRNA expression of IL-6, TNF-α and IL-1β as well as suppressed microglial infiltration, indicating that miR-216a-5p overexpression inhibited neuroinflammation. Besides, we found that miR-216a-5p upregulation inactivated the Wnt/β-catenin signaling pathway. Furthermore, KDM3A was the downstream target of miR-216a-5p and KDM3A knockdown attenuated neuropathic pain. Finally, through rescue assay, we found that KDM3A countervailed miR-216a-5p mediated regulation of neuropathic pain via the Wnt/β-catenin signaling pathway. To sum up, our study confirmed that miR-216a-5p alleviated neuropathic pain in rats by targeting KDM3A and inactivating the Wnt/β-catenin signaling pathway, which may open a new and useful way for treatment of neuropathic pain. |
| Databáze: | OpenAIRE |
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