Simultaneous Measurement of Serum Chemical Castration Agents and Testosterone Levels Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry
| Autor: | Sun Hee Jeon, Jin Young Kim, Hee Seung Kim, Junghan Song, Dae Hyun Ko, Kyunghoon Lee, Moon Kyo In, Sail Chun, Sang Hoon Song, Yeo-Min Yun |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Quality Control Health Toxicology and Mutagenesis Medroxyprogesterone Ion suppression in liquid chromatography–mass spectrometry Toxicology 01 natural sciences Analytical Chemistry Androgen deprivation therapy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Liquid chromatography–mass spectrometry Limit of Detection Tandem Mass Spectrometry medicine Androgen Receptor Antagonists Environmental Chemistry Humans Testosterone Castration Chemical castration Chromatography High Pressure Liquid Chemical Health and Safety Chromatography 010401 analytical chemistry Goserelin Acetate Cyproterone acetate Reproducibility of Results Reference Standards 0104 chemical sciences chemistry 030220 oncology & carcinogenesis Calibration medicine.drug |
| Zdroj: | Journal of analytical toxicology. 40(4) |
| ISSN: | 1945-2403 |
| Popis: | Chemical castration involves administration of drugs to prevent pathological sexual behavior, reduce abnormal sexual drive and treat hormone-dependent cancers. Various drugs have been used for chemical castration; however, substantial interindividual variability and side effects are often observed. In this study, we proposed a useful monitoring method for the application of chemical castration agents using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS). Testosterone, cyproterone acetate, medroxyprogesterone, goserelin acetate, leuprolide acetate and triptorelin acetate were analyzed by UPLC-MS-MS. The target drugs were extracted from serum samples by double protein precipitation using methanol. Testosterone-1,2-d2 and buserelin acetate were used as internal standards. Parameters of analytical performance were evaluated, including imprecision, linearity, ion suppression and detection capabilities. Testosterone measurements were compared with the results of immunoassays. Serum specimens from 51 subjects who underwent chemical castration were analyzed. All drugs and testosterone were well extracted and separated using our method. The method was essentially free from potential interferences and ion suppression. Within-run and between-run imprecision values were |
| Databáze: | OpenAIRE |
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