Identification of ATP Synthase As a Lipid Peroxide Protein Adduct in Pancreatic Islets From Humans With and Without Type 2 Diabetes Mellitus
| Autor: | M. A. Kendrick, Grzegorz Sabat, Annika Tibell, L. I. Szweda, C.-G. Östenson, Michael J. MacDonald, E.-C. Langberg |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
endocrine system
medicine.medical_specialty Lipid Peroxides endocrine system diseases Endocrinology Diabetes and Metabolism Clinical Biochemistry Type 2 diabetes medicine.disease_cause Kidney Biochemistry Lipid peroxidation Rats Sprague-Dawley chemistry.chemical_compound Islets of Langerhans Endocrinology Tandem Mass Spectrometry Internal medicine Diabetes mellitus medicine Animals Humans Cells Cultured geography geography.geographical_feature_category Lipid peroxide Pancreatic islets Biochemistry (medical) Type 2 Diabetes Mellitus JCEM Online: Brief Reports Mitochondrial Proton-Translocating ATPases medicine.disease Islet Mitochondria Rats Oxidative Stress medicine.anatomical_structure chemistry Diabetes Mellitus Type 2 Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Female Lipid Peroxidation Reactive Oxygen Species Oxidative stress |
| Popis: | Most current knowledge of pancreatic islet pathophysiology in diabetes mellitus has come from animal models. Even though islets from humans are readily available, only a few come from diabetic donors. We had the uncommon opportunity to acquire islets from humans with type 2 diabetes and used it to perform a study not previously done with human or animal islets.Oxidative stress has been proposed as a mechanism for impaired β-cell function in type 2 diabetes. Lipid peroxides caused by reactive oxygen species are damaging to body tissues. The objective was to determine whether lipid peroxide-protein adducts occur in pancreatic islets of humans with type 2 diabetes.Immunoblots with two antibodies to hydroxynonenal and 2 other antibodies we generated against reactive small aliphatic compounds were used to detect lipid peroxide-protein adducts in islets of patients with type 2 diabetes and controls.The antibodies reacted strongly to ≥5 islet proteins. The major hydroxynonenal adduct in the islets of type 2 diabetes patients was a 52-kDa protein seen with all 4 antibodies that was also seen in islets of nondiabetic humans, rat islets, and insulinoma cells and in mitochondria of various rat tissues. Nano-LC-MS/MS (liquid chromatography-tandem mass spectrometry) and MALDI-TOF (matrix-assisted laser desorption/ionization-time of flight) analysis identified the protein as the β-chain of the mitochondrial F-ATP synthase, an enzyme responsible for 95% of ATP formed in tissues.Lipid peroxide-protein adducts occur in β-cells in the nondiabetic state and in diabetes. Lipid peroxidation is thought to be damaging to tissues. Analogous to various other unhealthy characteristics, the presence in nondiabetic individuals of lipid peroxide-protein adducts does not necessarily indicate they are not detrimental. |
| Databáze: | OpenAIRE |
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