Orientation-dependent enhancement by H-NS of the activity of the type 1 fimbrial phase switch promoter in Escherichia coli
| Autor: | Peter Bjarke Olsen, Mark A. Schembri, Per Klemm |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Protein subunit Molecular Sequence Data Fimbria Regulator Biology medicine.disease_cause Polymerase Chain Reaction chemistry.chemical_compound Bacterial Proteins Transcription (biology) Escherichia coli Genetics medicine Promoter Regions Genetic Molecular Biology DNA Primers Phase variation Binding Sites Base Sequence Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition Molecular biology DNA-Binding Proteins chemistry Genes Bacterial Fimbriae Bacterial bacteria Fimbriae Proteins Lacz reporter Genes Switch DNA Protein Binding |
| Zdroj: | Molecular and General Genetics MGG. 259:336-344 |
| ISSN: | 1432-1874 0026-8925 |
| Popis: | Phase variation of type 1 fimbriation in Escherichia coli is associated with the inversion of a 314-bp DNA element, positioned proximal to and upstream of fimA, which encodes the major type 1 fimbrial subunit. This DNA switch region contains a promoter that drives transcription of fimA only when the switch is in the ON orientation. Using chromosomal and plasmid-borne lacZ reporter cassettes, we show here how the global regulator H-NS affects the activity of the fimA promoter. In phase-locked reporter cassettes the activity of the fimA promoter was found to be enhanced in a hns-positive background, but only when the switch was in the ON orientation. Also, the number of fimbriae produced by a phase-locked ON strain was significantly higher in a hns-positive background. By means of competitive gel retardation and DNase I protection assays, H-NS binding to DNA segments adjacent to and within the phase switch region was demonstrated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink