Resveratrol production in bioreactor: Assessment of cell physiological states and plasmid segregational stability
| Autor: | Filomena Silva, Fernanda C. Domingues, Margarida S. Afonso, Susana Ferreira |
|---|---|
| Přispěvatelé: | uBibliorum |
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Cell physiology endocrine system diseases lcsh:Biotechnology Cell Biology Resveratrol medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology Article Microbiology Flow cytometry 03 medical and health sciences chemistry.chemical_compound Plasmid 010608 biotechnology lcsh:TP248.13-248.65 medicine Bioreactor Bioprocess skin and connective tissue diseases Escherichia coli 030304 developmental biology 2. Zero hunger 0303 health sciences medicine.diagnostic_test Real-time qPCR organic chemicals food and beverages Flow Cytometry Plasmid stability medicine.anatomical_structure Biochemistry chemistry hormones hormone substitutes and hormone antagonists Biotechnology |
| Zdroj: | Biotechnology Reports Biotechnology Reports, Vol 5, Iss C, Pp 7-13 (2015) Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP |
| ISSN: | 2215-017X |
| Popis: | Highlights • Precursor concentration affected cell viability and resveratrol yields. • Cell viability and culture conditions influenced resveratrol yields. • Results show the relevance of process monitoring for bioprocess optimization. Resveratrol is a plant secondary metabolite commonly found in peanuts and grapevines with significant health benefits. Recombinant organisms can produce large amounts of resveratrol and, in this work, Escherichia coli BW27784 was used to produce resveratrol in bioreactors while monitoring cell physiology and plasmid stability through flow cytometry and real-time qPCR, respectively. Initially, the influence of culture conditions and precursor addition was evaluated in screening assays and the data gathered was used to perform the bioreactor assays, allowing the production of 160 μg/mL of resveratrol. Cellular physiology and plasmid instability affected the final resveratrol production, with lower viability and plasmid copy numbers associated with lower yields. In sum, this study describes new tools to monitor the bioprocess, evaluating the effect of culture conditions, and its correlation with cell physiology and plasmid segregational stability, in order to define a viable and scalable bioprocess to fulfill the need for larger quantities of resveratrol. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|