Metabolomic study of polyamines in rat urine following intraperitoneal injection of γ-hydroxybutyric acid
| Autor: | Sooyeun Lee, Moongi Ji, Young-A Kim, Boyeon Choi, Hyeon-Seong Lee, Chan Seo, Man-Jeong Paik, Meejung Park |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Endocrinology Diabetes and Metabolism medicine.medical_treatment Urinary system Clinical Biochemistry Intraperitoneal injection Hydroxybutyrates Spermine Urine Pharmacology 01 natural sciences Biochemistry Gas Chromatography-Mass Spectrometry Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Polyamines medicine Animals Metabolomics 030304 developmental biology 0303 health sciences 010401 analytical chemistry Metabolism Rats 0104 chemical sciences chemistry Putrescine Gas chromatography–mass spectrometry Polyamine Biomarkers Injections Intraperitoneal |
| Zdroj: | Metabolomics. 15 |
| ISSN: | 1573-3890 1573-3882 |
| DOI: | 10.1007/s11306-019-1517-2 |
| Popis: | Recently, illegal abuse of γ-hydroxybutyric acid (GHB) has increased in drug-facilitated crimes, but the determination of GHB exposure and intoxication is difficult due to rapid metabolism of GHB. Its biochemical mechanism has not been completely investigated. And a metabolomic study by polyamine profile and pattern analyses was not performed in rat urine following intraperitoneal injection with GHB. Urinary polyamine (PA) profiling by gas chromatography-tandem mass spectrometry was performed to monitor an altered PA according to GHB administration. Polyamine profiling analysis by gas chromatography–mass spectrometry combined with star pattern recognition analysis was performed in this study. The multivariate statistical analysis was used to evaluate discrimination among control and GHB administration groups. Six polyamines were determined in control, single and multiple GHB administration groups. Star pattern showed distorted hexagonal shapes with characteristic and readily distinguishable patterns for each group. N1-Acetylspermine (p |
| Databáze: | OpenAIRE |
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