Lipid peroxidation as pathway of aluminium cytotoxicity in human skin fibroblast cultures: Prevention by superoxide dismutase+catalase and vitamins E and C
| Autor: | E E Creppy, R Anane |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
0301 basic medicine
Antioxidant Cell Survival Health Toxicology and Mutagenesis medicine.medical_treatment Ascorbic Acid Oxidative phosphorylation In Vitro Techniques Toxicology medicine.disease_cause Lipid peroxidation Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Malondialdehyde medicine Humans Vitamin E Cells Cultured Skin 030102 biochemistry & molecular biology Super oxide dismutase biology Superoxide Dismutase Free Radical Scavengers General Medicine Fibroblasts Catalase chemistry Biochemistry 030220 oncology & carcinogenesis biology.protein Lipid Peroxidation Oxidative stress Aluminum |
| Zdroj: | Human & Experimental Toxicology. 20:477-481 |
| ISSN: | 1477-0903 0960-3271 |
| DOI: | 10.1191/096032701682693053 |
| Popis: | Lipid peroxidation is one of the main manifestations of oxidative damage and has been found to play an important role in the toxicity and carcinogenicity of many xenobiotics. In the present study, we investigated the possible induction of lipid peroxidation by aluminium in human foreskin fibroblast cultures by assaying the malondialde hyde (MDA) produced inside the cells. The MDA–thiobarbituric acid (TBA) adduct was assayed by HPLC using fluorometric quantification after extraction in n-butanol. Lactate dehydrogenase (LDH) release was used as a marker of aluminium toxicity. MDA production was significantly increased after 24 h incubation with aluminium and paralleled LDH release. Superoxide dismutase (SOD)+catalase and vitamins C and E added in the culture medium as oxygen radical and free radical scavengers were efficient in preventing MDA production by aluminium, indicating that oxidative processes are one of the main pathways whereby this metal induces cytotoxicity. The latter is also largely prevented, thus confirming the link between oxidative stress induced by aluminium and its cytotoxicity in human skin fibroblasts. Human & Experimental Toxicology (2001) 20, 477–481. |
| Databáze: | OpenAIRE |
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