Modular development of an inline monitoring system for waterborne pathogens in raw and drinking water
| Autor: | Thomas Westerhoff, Gerald Urban, Christian Heese, Verena Blättel, Dennis Elsässer, Silvia Vosseler, Michael Seidel, Matthias Hügle, Buren Scharaw, Andreas Kunze, Matthias Spoo, Reinhard Niessner, Daniel Karthe, Frank T. Hufert, Johannes Ho, Ole Behrmann, Gregory Dame, Andreas Tiehm |
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| Přispěvatelé: | Publica |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Decision support system media_common.quotation_subject Microorganism sample pretreatment Soil Science Indicator bacteria Water supply concentration system 010501 environmental sciences Biology 01 natural sciences Isothermal amplification 03 medical and health sciences Lab-on-a-Chip Hygiene Enumeration Environmental Chemistry Raw water hygiene monitoring 0105 earth and related environmental sciences Earth-Surface Processes Water Science and Technology media_common Global and Planetary Change business.industry drinking water Environmental engineering waterborne pathogens Geology Modular design raw water Live/dead discrimination Pollution PCR 030104 developmental biology Biochemical engineering business RPA |
| Zdroj: | Environmental Earth Sciences. 75 |
| ISSN: | 1866-6299 1866-6280 |
| Popis: | The state-of-the-art monitoring of drinking water hygiene is based on the cultivation and enumeration of indicator bacteria. Despite its proven reliability, this approach has the disadvantages of being (a) relatively slow and (b) limited to a small number of indicator bacteria. Ideally, alternative methods would be less time-consuming while providing information about a larger set of hygienically relevant microorganisms including viruses. In this paper, we present insights into the design of a modular concentration and detection system for bacteria, bacteriophages and viruses. Following further validation, this or similar techniques have the potential to extend and speed up the monitoring of raw and drinking water hygiene in the future. The system consists of different modules for the concentration of microorganisms, an amplification and detection unit that includes a module for the differentiation between live and dead microorganisms, and an automated system for decision support and self-diagnosis. The ongoing testing under controlled laboratory conditions and real-life conditions in the water supply industry yields further system improvements. Moreover, the increased sensitivity and broader range of microbiological parameters emphasize the need for a reconsideration of the currently used criteria for the assessment of (drinking) water hygiene. |
| Databáze: | OpenAIRE |
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