GS15, a 15-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) homologous to rbet1
Autor: | Wanjin Hong, Siew Heng Wong, Yue Xu, V. N. Subramaniam, Tong Zhang |
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Rok vydání: | 1997 |
Předmět: |
Molecular Sequence Data
Vesicular Transport Proteins Golgi Apparatus Biology Biochemistry symbols.namesake Animals Amino Acid Sequence Qc-SNARE Proteins RNA Messenger Fluorescent Antibody Technique Indirect Molecular Biology Peptide sequence COPII Integral membrane protein Secretory pathway Glutathione Transferase Base Sequence Endoplasmic reticulum Sulfhydryl Reagents Membrane Proteins Cell Biology COPI Golgi apparatus Cell biology Rats Vesicular transport protein Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins Solubility Ethylmaleimide symbols Carrier Proteins SNARE Proteins |
Zdroj: | The Journal of biological chemistry. 272(32) |
ISSN: | 0021-9258 |
Popis: | Bet1p plays an essential role in vesicular transport from the endoplasmic reticulum (ER) to the Golgi in yeast, and it functions as a vesicle soluble N-ethylmaleimide-sensitive factor protein receptor (v-SNARE). A mammalian protein related to Bet1p has been reported previously and was referred to as rbet1. We have now identified a new mammalian protein that is homologous to rbet1 (28% amino acid identity). mRNA for this rbet1 homologue is widely expressed in rat tissues. Affinity-purified polyclonal antibodies raised against recombinant protein specifically recognized a 15-kilodalton integral membrane protein highly enriched in Golgi membranes. Indirect immunofluorescence microscopy revealed that this protein is specifically associated with the Golgi apparatus in diverse cell types. Biochemical characterization established that this protein behaves like a SNARE and was named GS15 (Golgi SNARE with a size of 15 kilodaltons). These properties raise the possibility that GS15 is a novel SNARE mediating a yet to be defined transport event associated with the Golgi apparatus. |
Databáze: | OpenAIRE |
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