Analysis of Autoantibodies Related to Tumor Progression in Sera from Patients with High-grade Non-muscle-invasive Bladder Cancer
| Autor: | Makoto Saegusa, Benio Tsuchiya, Sho Minami, Masatsugu Iwamura, Shi-Xu Jiang, Naoki Goshima, Eriko Fukuda, Kazumasa Matsumoto, Daisuke Hagiuda, Ryo Nagashio, Manabu Hattori, Yuichi Sato, Kazuo Hachimura |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Proteomics Cancer Research Pathology medicine.medical_specialty Proteome Lymphovascular invasion Dot blot Lesion 03 medical and health sciences Protein Phosphatase 1 Medicine Humans Pathological Aged Autoantibodies Aged 80 and over Bladder cancer business.industry Autoantibody General Medicine Middle Aged medicine.disease Prognosis Immunohistochemistry 030104 developmental biology Oncology Urinary Bladder Neoplasms Tumor progression Immunoglobulin G Disease Progression Biomarker (medicine) Female medicine.symptom Neoplasm Grading business |
| Zdroj: | Anticancer research. 37(12) |
| ISSN: | 1791-7530 |
| Popis: | BACKGROUND/AIM Bladder cancer (BC) has a high recurrence rate and may progress to being a muscle-invasive lesion, that is potentially associated with a poor prognosis. We identified tumor-associated proteins that were recognized by autoantibodies in sera from patients with high-grade non-muscle-invasive bladder cancer (HG-NMIBC) by proteomic analysis. MATERIALS AND METHODS The serum levels of these autoantibodies against identified proteins were validated by dot blot analysis with sera from 95 patients with BC and 35 healthy controls. The expression of identified proteins was immunohistochemically analyzed in 115 BC tissues. RESULTS Autoantibody against protein phosphatase 1, catalytic subunit, alpha isoform (PPP1CA) protein was detected in pretreated sera from patients with HG-NMIBC who showed progression. The serum IgG level of anti-PPP1CA autoantibody was significantly correlated with pathological stage, grade, lymphovascular invasion, and prognosis. The immunoreactions for PPP1CA protein in BC was significantly correlated with pathological stage, grade, and lymphovascular invasion. CONCLUSION PPP1CA is a candidate sero-diagnostic and prognostic marker for patients with BC. |
| Databáze: | OpenAIRE |
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