Previous estradiol treatment during midlife maintains transcriptional regulation of memory-related proteins by ERα in the hippocampus in a rat model of menopause

Autor: Nina E. Baumgartner, Katelyn L. Black, Jill M. Daniel, Shannon M. McQuillen
Rok vydání: 2021
Předmět:
0301 basic medicine
Aging
medicine.medical_specialty
Transcription
Genetic
medicine.drug_class
Ovariectomy
Hippocampus
Gene Expression
Estrogen receptor
Biology
Hippocampal formation
Article
03 medical and health sciences
0302 clinical medicine
Memory
Internal medicine
Gene expression
medicine
Transcriptional regulation
Animals
Hippocampus (mythology)
Rats
Long-Evans
Estradiol
business.industry
Brain-Derived Neurotrophic Factor
General Neuroscience
Estrogen Receptor alpha
medicine.disease
Menopause
030104 developmental biology
Endocrinology
Estrogen
Models
Animal
Ovariectomized rat
Female
Neurology (clinical)
Geriatrics and Gerontology
business
Disks Large Homolog 4 Protein
Estrogen receptor alpha
hormones
hormone substitutes
and hormone antagonists
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Neurobiol Aging
ISSN: 0197-4580
DOI: 10.1016/j.neurobiolaging.2021.05.022
Popis: Previous midlife estradiol treatment, like continuous treatment, improves memory and results in lasting increases in hippocampal levels of estrogen receptor (ER) α and ER-dependent transcription in ovariectomized rodents. We hypothesized that previous and continuous midlife estradiol act to specifically increase levels of nuclear ERα, resulting in transcriptional regulation of proteins that mediate estrogen effects on memory. Ovariectomized middle-aged rats received estradiol or vehicle capsule implants. After 40 days, rats initially receiving vehicle received another vehicle capsule (Vehicle). Rats initially receiving estradiol received either another estradiol (Continuous Estradiol) or a vehicle (Previous Estradiol) capsule. One month later, hippocampal genes and proteins were analyzed. Continuous and previous estradiol increased levels of nuclear, but not membrane or cytosolic ERα and had no effect on Esr1. Continuous and previous estradiol impacted gene expression and/or protein levels of mediators of estrogenic action on memory including ChAT, BDNF, and PSD-95. Findings demonstrate a long-lasting role for hippocampal ERα as a transcriptional regulator of memory following termination of previous estradiol treatment in a rat model of menopause.
Databáze: OpenAIRE
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