Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway
| Autor: | Annette A. Alcasabas, Jeffrey B. Bachant, Brian A. Desany, Stephen J. Elledge |
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| Rok vydání: | 1998 |
| Předmět: |
DNA Replication
Saccharomyces cerevisiae Proteins Transcription Genetic DNA repair Genes Fungal Gene Expression Mitosis Cell Cycle Proteins Eukaryotic DNA replication Saccharomyces cerevisiae Protein Serine-Threonine Kinases Biology S Phase Fungal Proteins DNA replication factor CDT1 Control of chromosome duplication Ribonucleotide Reductases Genetics Hydroxyurea Point Mutation DNA Fungal Checkpoint Kinase 2 Intracellular Signaling Peptides and Proteins G2-M DNA damage checkpoint Cell biology DNA replication checkpoint biology.protein Origin recognition complex Protein Kinases Gene Deletion Research Paper DNA Damage Developmental Biology |
| Zdroj: | Genes & Development. 12:2956-2970 |
| ISSN: | 1549-5477 0890-9369 |
| Popis: | RAD53 and MEC1 are essential genes required for the transcriptional and cell cycle responses to DNA damage and DNA replication blocks. We have examined the essential function of these genes and found that their lethality but not their checkpoint defects can be suppressed by increased expression of genes encoding ribonucleotide reductase. Analysis of viable null alleles revealed that Mec1 plays a greater role in response to inhibition of DNA synthesis than Rad53. The loss of survival in mec1 and rad53 null or point mutants in response to transient inhibition of DNA synthesis is not a result of inappropriate anaphase entry but primarily to an inability to complete chromosome replication. We propose that this checkpoint pathway plays an important role in the maintenance of DNA synthetic capabilities when DNA replication is stressed. |
| Databáze: | OpenAIRE |
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