Characterization of non-producer human cells induced by Kirsten sarcoma virus
| Autor: | Robert J. Huebner, Walter A. Nelson-Rees, Han Y. Cho, Paul Arnstein, Raymond V. Gilden, J. S. Rhim, Mina Lee Vernon |
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| Rok vydání: | 1975 |
| Předmět: |
Cancer Research
Virus Cultivation DNA polymerase viruses Transplantation Heterologous Viral transformation In Vitro Techniques Feline leukemia virus Virus Cell Line Inclusion Bodies Viral Mice Antigen medicine Animals Humans Antigens Viral Osteosarcoma biology Complement Fixation Tests DNA-Directed RNA Polymerases medicine.disease biology.organism_classification Virology Rats Titer Leukemia Cell Transformation Neoplastic Oncology Helper virus DNA Nucleotidyltransferases biology.protein Sarcoma Experimental Gammaretrovirus Neoplasm Transplantation |
| Zdroj: | International journal of cancer. 16(5) |
| ISSN: | 0020-7136 |
| Popis: | Non-producer (NP) human cells induced by the Kirsten sarcoma virus were characterized. These morphologically altered NP cells produced neither infectious virus nor complement-fixing antigens of the murine sarcoma-leukemia virus complex. The NP cells did not release RNA-dependent DNA polymerase and type-C virus particles with a density of approximately 1.15 g/ml in sucrose gradients by 3H-uridine labelling. The NP cells produced tumors when transplanted subcutaneously into athymic nude mice. The tumor cells re-established in culture resembled the orginal NP cells, were confirmed as human cells by karyological analysis and were also found to be "non-producer". The sarcoma virus genome in NP cells could be rescued not only by co-cultivation with "helper virus"-releasing cells but also by superinfection with helper type-C viruses. Murine (Rauscher, Ki-MuLV, AT-124 and two other xenotropic viruses), feline, RD-114 and Simian (woolly monkey and baboon) type-C viruses possessed the ability to rescue the sarcoma genome from NP cells but not AKR leukemia virus. In addition, the feline leukemia virus titer obtained by the rescuing technique in NP cells was the same as those obtained in feline embryo and NP cells by CF induction assay. |
| Databáze: | OpenAIRE |
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