Correlation of clinical data with fallopian tube specimen immune cells and tissue culture capacity
| Autor: | Katie M. Smith, Naveena B. Janakiram, Ankita Raman, Coralee Toal, Doris M. Benbrook, Satish Kumar Ramraj |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Pathology medicine.medical_specialty Cell type medicine.medical_treatment Fimbria Cell Culture Techniques Carcinoma Ovarian Epithelial Biology Article Cryopreservation Specimen Handling Salpingectomy 03 medical and health sciences Tissue culture 0302 clinical medicine Immune system Cell Line Tumor medicine Humans Neoplasms Glandular and Epithelial Fallopian Tubes Aged Ovarian Neoplasms Epithelial Cells Cell Biology General Medicine Middle Aged medicine.disease 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Female Ovarian cancer Developmental Biology Fallopian tube |
| Zdroj: | Tissue and Cell. 52:57-64 |
| ISSN: | 0040-8166 |
| DOI: | 10.1016/j.tice.2018.04.001 |
| Popis: | Human fallopian tube fimbria secretory epithelial cells (hFTSECs) are considered an origin of ovarian cancer and methods for their culture from fallopian tube specimens have been reported. Our objective was to determine whether characteristics of the donors or surgeries were associated with the capacities of fimbria specimens to generate hFTSEC cultures or their immune profiles. There were no surgical complications attributable to fallopian tube removal. Attempts to establish primary hFTSEC cultures were successful in 37 of 55 specimens (67%). Success rates did not differ significantly between specimens grouped by patient or surgery characteristics. Established cultures could be revived after cryopreservation and none became contaminated with microorganisms. Two cultures evaluated for long term growth senesced between passages 10 and 15. M1 macrophages were the predominant cell type, while all other immune cells were present at much lower percentages. IL-10 and TGF-β exhibited opposing trends with M1 and M2 macrophages. Plasma IL-10 levels exhibited significant positive correlation with patient age. In conclusion, fallopian tube fimbria specimens exhibit a pro-inflammatory phenotype and can be used to provide a source of hFTSECs that can be cultured for a limited time regardless of the donor patient age or race, or the type of surgery performed. |
| Databáze: | OpenAIRE |
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