Scaffold-guided bone regeneration in large volume tibial segmental defects
| Autor: | Stephanie Fountain, Jan Henkel, Roland Steck, Devakar Epari, Arne Berner, Mark Knackstedt, Michael Schuetz, Siamak Saifzadeh, Dietmar W. Hutmacher, Maria A. Woodruff, Flavia Medeiros Savi |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Scaffold Bone Regeneration Histology Physiology Endocrinology Diabetes and Metabolism Dynamic compression plate Pilot Projects 030209 endocrinology & metabolism Bone morphogenetic protein medicine.disease_cause Bone and Bones Bone tissue engineering Weight-bearing 03 medical and health sciences 0302 clinical medicine Animals Medicine Bone regeneration 030304 developmental biology 0303 health sciences Sheep Tibia Tissue Engineering Tissue Scaffolds business.industry business Biomedical engineering Large animal Volume (compression) |
| Zdroj: | Bone. 153:116163 |
| ISSN: | 8756-3282 |
| DOI: | 10.1016/j.bone.2021.116163 |
| Popis: | Large volume losses in weight bearing long bones are a major challenge in clinical practice. Despite multiple innovations over the last decades, significant limitations subsist in current clinical treatment options which is driving a strong clinical demand for clinically translatable treatment alternatives, including bone tissue engineering applications. Despite these shortcomings, preclinical large animal models of large volume segmental bone defects to investigate the regenerative capacity of bone tissue engineering strategies under clinically relevant conditions are rarely described in literature. We herein present a newly established preclinical ovine animal model for the treatment of XL volume (19 cm3) segmental tibial defects. In eight aged male Merino sheep (age > 6 years) a mid-diaphyseal tibial segmental defect was created and stabilized with a 5.6 mm Dynamic Compression Plate (DCP). We present short-term (3 months) and long-term (12–15 months) results of a pilot study using medical grade Polycaprolactone-Tricalciumphosphate (mPCL-TCP) scaffolds combined with a dose of 2 mg rhBMP-7 delivered in Platelet-Rich- Plasma (PRP). Furthermore, detailed analyses of the mechanical properties of the scaffolds as well as interfragmentary movement (IFM) and DCP-surface strain in vitro and a comprehensive description of the surgical and post-surgery protocol and post-mortem analysis is given. |
| Databáze: | OpenAIRE |
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