EGFR expression is linked to osteopontin and Nf-κB signaling in clear cell renal cell carcinoma
| Autor: | Dora Fabbro, Ita Hadžisejdić, Nives Jonjić, Manuela Avirović, Blaženka Grahovac, Maja Grahovac, Giuseppe Damante, Ksenija Lučin, Gordana Đorđević, Koviljka Matušan-Ilijaš |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
medicine.medical_specialty Real-Time Polymerase Chain Reaction medicine.disease_cause Receptor tyrosine kinase stomatognathic system Cell Line Tumor Internal medicine Gene expression Humans Renal cell carcinoma EGFR Nuclear factor kappa B Osteopontin Tissue array analysis Medicine RNA Messenger Epidermal growth factor receptor Carcinoma Renal Cell biology business.industry NF-kappa B carcinoma renal cell Nuclear Factor kappa B osteopontin tissue array analysis General Medicine medicine.disease Immunohistochemistry Kidney Neoplasms Up-Regulation ErbB Receptors Clear cell renal cell carcinoma Endocrinology Oncology biology.protein Cancer research Cyclin-dependent kinase 8 Signal transduction business Carcinogenesis Signal Transduction |
| Zdroj: | Clinical and Translational Oncology. 15:65-71 |
| ISSN: | 1699-3055 1699-048X |
| DOI: | 10.1007/s12094-012-0889-9 |
| Popis: | Aim and background: Epidermal growth factor receptor(EGFR) is a receptor tyrosine kinase involved in many important aspects of cell biology that are related to tumorigenesis. There are opposite evidences of the role of EGFR in renal cancer and the outcome of EGFR-targeted therapies, suggesting the complexity of EGFR signaling pathways. In vitro, osteopontin (OPN) and nuclear factor kappa B (NF-kB) are thought to be involved in specific ligand-independent EGFR activation that could have a role in resistance to EGFR mAb therapy. Aim of this study was to analyze the relationship between EGFR and OPN at the protein and mRNA level, as well as their relation to NF-kB in clear cell renal cell carcinoma (CCRCC). Materials and methods: Expression of EGFR, OPN, and p65 NF-kB protein was analyzed using immunohistochemistry and compared mutually in 88 CCRCC samples. Expression of EGFR and OPN mRNAs was analyzed using quantitative Real-time PCR in 22 CCRCC samples and compared mutually and with NF-kB protein expression. Results: Epidermal growth factor receptor mRNA level was higher in CCRCC samples in comparison with normal renal tissue (p=0.012) and was associated with high OPN mRNA level, and with NF-kB activation (p |
| Databáze: | OpenAIRE |
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