Effect of topical ozonated sunflower oil on second intention wound healing in turtles: a randomised experimental study
| Autor: | Rosario Lucena, Pedro J. Ginel, Elena Mozos, Rafael Guerra, J. Negrini, María Teresa Ruiz-Campillo |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
chelonians food.ingredient Skin wound 040301 veterinary sciences Secondary infection Administration Topical Dermatology Gastroenterology 0403 veterinary science 03 medical and health sciences food Collagen fibres Internal medicine medicine Animals Sunflower Oil Clinical significance 030304 developmental biology Skin 0303 health sciences Wound Healing General Veterinary integumentary system business.industry Sunflower oil 04 agricultural and veterinary sciences Antimicrobial Turtles ozone Trachemys scripta Animals Zoo Female Original Article Wound healing business |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X |
| Popis: | BACKGROUND Ozone is an antimicrobial agent that in experimental and case-control studies has been found to exert a positive effect on wound healing. Wild and pet chelonians frequently present insidious wounds exhibiting secondary infections and/or delayed healing. OBJECTIVES Evaluate the effects of topical ozonated sunflower oil on second-intention healing of acute experimental skin wounds in red-eared sliders (Trachemys scripta elegans). METHODS Randomised within-subject controlled study; Group 1 (n = 24) was used to assess clinical healing features; Group 2 (n = 12) was used for histological evaluation in which two sets of wounds were biopsied at 2, 7, 14, 21, 28 and 42 days over the course of the cicatrisation process. A single 6 mm diameter wound was made on each rear limb and topical ozonated (950 peroxide value) and non-ozonated sunflower oil were applied daily for one week on treated and contralateral control wounds, respectively. RESULTS Mean wound size was significantly lower in the ozone-treated group at day 28 (p < 0.0001) with differences of clinical relevance (74.04% vs. 93.05% reduction of initial wound size). Histologically, the acute inflammatory reaction was enhanced in treated wounds, with significantly higher numbers of heterophils (p = 0.0016), lymphocytes (p < 0.001) and fibroblasts (p < 0.001). CONCLUSIONS Daily topical application of ozonated sunflower oil over the course of one week improved the healing of acute, full-thickness skin wounds in chelonians. This clinical outcome was histologically correlated with an enhanced acute inflammatory reaction, as well as the production and remodelling of collagen fibres. |
| Databáze: | OpenAIRE |
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