Proteomic characterization of kefir milk by two-dimensional electrophoresis followed by mass spectrometry
| Autor: | Francesca Bonazza, Silvia Vincenzetti, Stefania Silvi, Stefania Pucciarelli, Giuseppe Santini, Valeria Polzonetti, Massimo Ricciutelli, Yulia Klimanova, Paolo Polidori |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
01 natural sciences Mass Spectrometry chemistry.chemical_compound Kefir fluids and secretions Nutraceutical Animals Electrophoresis Gel Two-Dimensional Food science Acetic acid bacteria Spectroscopy chemistry.chemical_classification Organic Agriculture biology 010405 organic chemistry Chemistry 010401 analytical chemistry Caseins food and beverages Kefiran Milk Proteins biology.organism_classification Antimicrobial 0104 chemical sciences Amino acid Lactic acid Fermentation Peptides |
| Zdroj: | Journal of Mass Spectrometry. :e4635 |
| ISSN: | 1076-5174 |
| DOI: | 10.1002/jms.4635 |
| Popis: | Kefir is a type of fermented milk obtained thanks to the introduction of "kefir grains" in mammalian milk. Kefir grains consist of lactic and acetic acid bacteria and yeasts in alternative proportions that are held together by a matrix of complex sugars known as "kefiran." Thanks to the fermentative process, the kefir milk is rich in nutraceutical substances such as amino acids, vitamins, and mineral salts. The most valuable compounds of kefir fermentation are mainly lactic acid, exopolysaccharides, and bioactive peptides, the resulting products of proteolytic release from milk proteins (caseins and whey proteins). Among the nutraceutical properties of kefir are antimicrobial and antitumor activity, immunomodulating effect, and cholesterol-lowering effect. Therefore, in light of these intriguing properties of kefir milk, in this work, a proteomic analysis, by two-dimensional electrophoresis followed by mass spectrometry, has been performed. As a result, milk-derived polypeptides were identified in commercial kefir milk from organic farming. In particular, polypeptides deriving from κ-, αs1 -, and αs2 -caseins that may have potentially beneficial effects on human health have been detected. |
| Databáze: | OpenAIRE |
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