Multiple Non-reducing Chain Termini Isolated from Bovine Corneal Keratan Sulfates
| Autor: | Gui-Hua Tai, Thomas N. Huckerby, Ian A. Nieduszynski |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Magnetic Resonance Spectroscopy
Stereochemistry Keratan sulfate Sequence analysis Molecular Sequence Data Oligosaccharides Biochemistry Cornea chemistry.chemical_compound Sugar Alcohols Molecular size Acetylglucosaminidase Animals Molecular Biology chemistry.chemical_classification biology Chemistry Age Factors Cell Biology Nuclear magnetic resonance spectroscopy Oligosaccharide Chromatography Ion Exchange Enzyme Carbohydrate Sequence Proteoglycan Keratan Sulfate Chromatography Gel biology.protein Cattle Keratanase II Oxidation-Reduction Sequence Analysis |
| Zdroj: | Journal of Biological Chemistry. 271:23535-23546 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.271.38.23535 |
| Popis: | Keratan sulfate-containing proteoglycans were isolated from bovine cornea (15-month-old to 3-year-old animals) and digested with the enzyme, keratanase II. The released oligosaccharides, which included non-reducing termini and repeat region oligosaccharides but not linkage regions, were reduced with alkaline borohydride and fractionated on a Spherisorb column. These oligosaccharides were examined by 600-MHz 1H NMR spectroscopy using one- and two-dimensional methods and, in addition to some oligosaccharide alditols previously recovered from skeletal keratan sulfate, the following new capping structures were identified: NeuAcalpha2-6Galbeta1-4GlcNAc(S)-ol, NeuAcalpha2-3Gal(S)beta1-4GlcNAc(S)beta1-3Galbeta1-4GlcNAc(S )-ol, NeuGcalpha2-6Galbeta1-4GlcNAc(S)beta1-3Galbeta1-4Gl cNA c(S)-ol, NeuGcalpha2-3Galbeta1-4GlcNAc(S)beta1-3Galbeta1-4Gl cNA c(S)-ol, NeuGcalpha2-3Gal(S)beta1-4GlcNAc(S)beta1-3Galbeta1-4GlcNAc(S )-ol, NeuGcalpha2-3Gal(S)beta1-4GlcNAc(S)beta1-3Gal(S)beta1-4GlcNAc(S)-o l, Galalpha1-3Galbeta1-4GlcNAc(S)beta1-3Galbeta1-4GlcNAc( S)-ol, Galalpha1-3Galbeta1-4GlcNAc(S)beta1-3Gal(S)beta1-4GlcNAc(S)- ol, GlcNAc(S)beta1-3Gal(S)beta1-4GlcNAc(S)-ol, and GalNAc(S)beta1-3Gal(S)beta1-4GlcNAc(S)-ol. These structures represent seven families of capping residues, whose relative molar proportions are given in parentheses: NeuAcalpha(2-3)- (12%), NeuAcalpha(2-6)- (41%), NeuGcalpha(2-3)- and NeuGcalpha(2-6)- families (12%), Galalpha(1-3)- (26%), GalNAc(S)beta(1-3)- (5%), and GlcNAc(S)beta(1-3)- (4%). It is not clear, at present, where each of these structures occurs on the bi-antennary N-linked corneal keratan sulfate chains, which themselves occur within three keratan sulfate proteoglycan species. However, examination of the relative proportions of the capping to the repeat structures and knowledge of the average molecular size suggests that the sum of these non-reducing termini represents the caps of two antennae. |
| Databáze: | OpenAIRE |
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