Adenosine triphosphate, polymyxin B and B16 cell-derived immunization induce anticancer response
| Autor: | Claudia Robles-Planells, Tanya Neira, Leonel E. Rojo, Alejandro Escobar, Claudio Acuña-Castillo, Claudio Cappelli, Ximena Lopez, Javier Mena, Luis A. Milla, Mónica Imarai, Carlos Barrera-Avalos, Elías Leiva-Salcedo, Ricardo Fernández |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Immunology Cell Melanoma Experimental Cancer Vaccines Mice 03 medical and health sciences Adenosine Triphosphate 0302 clinical medicine Immune system Phagocytosis Antigen Antigens Neoplasm In vivo Tumor Cells Cultured medicine Alarmins Animals Immunology and Allergy CD40 Antigens Polymyxin B Antigen Presentation Chemistry Dendritic Cells Immunotherapy In vitro Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research Cytokines Immunization Spleen CD8 medicine.drug |
| Zdroj: | Immunotherapy. 13:309-326 |
| ISSN: | 1750-7448 1750-743X |
| Popis: | Aim: Whole dead tumor cells can be used as antigen source and the induction of protective immune response could be enhanced by damage-associated molecular patterns. Materials & methods: We generated whole dead tumor cells called B16-immunogenic cell bodies (ICBs) from B16 melanoma cells by nutrient starvation and evaluated the in vivo antitumor effect of B16-ICBs plus ATP and polymyxin B (PMB). Results: The subcutaneous immunization with B16-ICBs + PMB + ATP a 50% of tumor-free animals and induced a significant delay in tumor growth in a prophylactic approach. These results correlated with maturation of bone marrow-derived dendritic cells and activation of T CD8+lymphocytes in vitro. Conclusion: Altogether, ICB + ATP + PMB is efficient in inducing the antitumor efficacy of the whole dead tumor cells vaccine. |
| Databáze: | OpenAIRE |
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