Immunohistochemistry forBRAFV600E in the Differential Diagnosis of Hairy Cell Leukemia vs Other Splenic B-Cell Lymphomas
| Autor: | Christopher Lanigan, James R. Cook, Samir K. Turakhia, Steven H. Swerdlow, Raymond R. Tubbs, Fatima Hamadeh |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Proto-Oncogene Proteins B-raf Pathology medicine.medical_specialty Lymphoma B-Cell Chronic lymphocytic leukemia DNA Mutational Analysis Biology Polymerase Chain Reaction Diagnosis Differential medicine Humans Hairy cell leukemia B-cell lymphoma B cell Aged Leukemia Hairy Cell General Medicine Middle Aged Plasma cell neoplasm medicine.disease Immunohistochemistry Lymphoma medicine.anatomical_structure Mutation Cancer research Female Differential diagnosis |
| Zdroj: | American Journal of Clinical Pathology. 144:87-93 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1309/ajcp5wvxj2ktlodo |
| Popis: | Objectives: Recent reports have used immunohistochemistry (IHC) with a mutation-specific antibody to detect the BRAF V600E mutation, which is found in nearly all cases of hairy cell leukemia (HCL). To date, however, only a small number of non-HCL, splenic B-cell lymphomas have been examined by IHC. Methods: We analyzed 121 cases, including 26 HCLs, 52 non-HCL splenic lymphomas, 22 chronic lymphocytic leukemias/small lymphocytic lymphomas (CLLs/SLLs), and 21 plasma cell neoplasms (PCNs) for BRAF V600E expression by IHC. Molecular testing for BRAF V600E was performed in a subset of cases, using allele-specific polymerase chain reaction and/or Sanger sequencing. Results: Twenty-six (100%) of 26 HCL cases were positive by IHC vs one (1%) of 95 non-HCL cases. Positive staining was identified in one (2%) of 44 splenic marginal zone lymphomas (SMZLs), while each of 22 CLLs/SLLs, 21 PCNs, six unclassifiable splenic lymphomas, and two HCL variants were negative. IHC and molecular results were concordant in all cases examined (21 HCLs and 21 non-HCLs, including the BRAF+ SMZLs). Conclusions: The detection of BRAF V600E by IHC is useful in the distinction of HCLs from other splenic-based lymphomas, although the identification of at least rare SMZLs containing this abnormality illustrates the continuing need for a multiparameter approach to diagnosis. |
| Databáze: | OpenAIRE |
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