Factors mediating lipofection potency of a series of cationic phosphonolipids in human cell lines
| Autor: | Jean-Jacques Yaouanc, Dimitris Loukopoulos, J.‐C. Clement, Panagoula Kollia, Daphne Koumbi, Zili Sideratou |
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| Přispěvatelé: | Department of Genetics and Biotechnology, Faculty of Biology, School of Physical Sciences, National and Kapodistrian University of Athens (NKUA), First Department of Medicine, University of Athens Medical School [Athens], Chimie, Electrochimie Moléculaires et Chimie Analytique (CEMCA), Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institute of Physical Chemistry ( NCSR ), Bioinformatics and Medical Informatics Team, Biomedical Research Foundation of the Academy of Athens, Athens, Greece |
| Rok vydání: | 2006 |
| Předmět: |
DNA incorporation
Cationic phosphonolipid [SDV]Life Sciences [q-bio] Transgene Cell Biophysics 02 engineering and technology Biology Transfection Biochemistry 03 medical and health sciences Cations Cell Line Tumor medicine Humans [CHIM]Chemical Sciences Transgene expression Nuclear Cationic liposome Gene transfer Molecular Biology Phospholipids 030304 developmental biology 0303 health sciences Liposome Nucleic Acid Hybridization 021001 nanoscience & nanotechnology Molecular biology In vitro Cell biology Kinetics medicine.anatomical_structure Lipofectamine 0210 nano-technology Lipofection condition Plasmids K562 cells |
| Zdroj: | Biochimica et Biophysica Acta (BBA)-General Subjects Biochimica et Biophysica Acta (BBA)-General Subjects, Elsevier, 2006, 1760 (8), pp.1151-1159. ⟨10.1016/j.bbagen.2006.03.005⟩ |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2006.03.005 |
| Popis: | International audience; A series of cationic liposomes known as cationic phosphonolipids (CPs) were evaluated as vehicles for in vitro gene transfer in K562 erythroleukemia cells and 5637 epithelial carcinoma cells. For each CP and target cell type examined, detailed analyses were performed to determine optimal transfection conditions (lipid/ DNA (+/−) charge ratio, amount of complexed episomal DNA, liposomal and lipoplex size, complexation medium and duration of complex-cell exposure time). Lipofection conditions were determined to be both cell- and lipid-type specific. Complexation medium critically affected transfection competence. The initial size of the liposome was not always predictive of lipofection potency. The lipid chemical composition had a strong impact upon lipofection efficiency; DOPE inclusion in the liposome formulations was found to affect the levels of transgene expression in a cell-dependent way. Notably, effective transgene expression was characterized by prominent plasmid nuclear incorporation. Human Aγ- and ε-globin transgene nuclear incorporation and expression in 5637 cells post GLB.391-mediated lipofection lends credence to its use as a vehicle of therapeutic transgene delivery. |
| Databáze: | OpenAIRE |
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