Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference
| Autor: | Michael E. Breimer, Bo E. Samuelsson, Anita Jacobsson, Lola Svensson, Jan Holgersson, Ami Ulfvin |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Blood Platelets
Isoantigens Blood Component Transfusion Biochemistry Epitope ABO Blood-Group System chemistry.chemical_compound Glycolipid Antigen Western blot medicine Humans Platelet Molecular Biology Glycoproteins chemistry.chemical_classification biology medicine.diagnostic_test Cell Biology Glycosphingolipid Molecular biology chemistry biology.protein Chromatography Thin Layer Glycolipids Antibody Glycoprotein |
| Zdroj: | Glycoconjugate Journal. 7:601-608 |
| ISSN: | 1573-4986 0282-0080 |
| DOI: | 10.1007/bf01189080 |
| Popis: | Total non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region. |
| Databáze: | OpenAIRE |
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