Tuning Nanopore Diameter of Titanium Surfaces to Improve Human Gingival Fibroblast Response

Autor: Maria Del Mar Ferrà-Cañellas, Joana M. Ramis, Marta Monjo, Maria Antonia Llopis-Grimalt
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Scanning electron microscope
medicine.medical_treatment
humanos
Gingiva
propiedades de superficie
02 engineering and technology
Electrolyte
lcsh:Chemistry
Contact angle
Nanopores
0302 clinical medicine
Materials Testing
Dental implant
lcsh:QH301-705.5
Spectroscopy
Cells
Cultured
Titanium
Nanoporous
General Medicine
titanio
021001 nanoscience & nanotechnology
Computer Science Applications
soft tissue integration
Nanopore
encía
nanoporos
0210 nano-technology
proliferación celular
Materials science
Surface Properties
Cells
electrochemical anodization
chemistry.chemical_element
adhesión celular
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
medicine
Cell Adhesion
Humans
Physical and Theoretical Chemistry
perfiles de expresión génica
Molecular Biology
Cell Proliferation
Anodizing
Gene Expression Profiling
Organic Chemistry
nanopore diameter
030206 dentistry
surface area
Fibroblasts
análisis de materiales
fibroblastos
lcsh:Biology (General)
lcsh:QD1-999
chemistry
células
Biomedical engineering
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 19, Iss 10, p 2881 (2018)
Volume 19
Issue 10
ISSN: 1422-0067
Popis: The aim of this study was to determine the optimal nanopore diameter of titanium nanostructured surfaces to improve human gingival fibroblast (hGF) response, with the purpose of promoting gingiva integration to dental implant abutments. Two TiO2 nanoporous groups with different diameters (NP-S 48 nm and NP-B 74 nm) were grown on Ti foils using an organic electrolyte containing fluoride by electrochemical oxidation, varying the applied voltage and the interelectrode spacing. The surfaces were characterized by scanning electron microscope (SEM), atomic force microscopy (AFM), and contact angle. The hGF were cultured onto the different surfaces, and metabolic activity, cytotoxicity, cell adhesion, and gene expression were analyzed. Bigger porous diameters (NP-B) were obtained by increasing the voltage used during anodization. To obtain the smallest diameter (NP-S), apart from lowering the voltage, a lower interelectrode spacing was needed. The greatest surface area and number of peaks was found for NP-B, despite these samples not being the roughest as defined by R-a. NP-B had a better cellular response compared to NP-S. However, these effects had a significant dependence on the cell donor. In conclusion, nanoporous groups with a diameter in the range of 74 nm induce a better hGF response, which may be beneficial for an effective soft tissue integration around the implant.
This research was funded by the Osteology Foundation (13-069), the Ministerio de Educacion Cultura y Deporte (contract to M.A.L.-G; FPU15/03412), the Instituto de Salud Carlos III (contract to J.M.R; CP16/00124), and the Ministerio de Empleo y Seguridad Social with the Sistema de Garantia Juvenil (contract to M.d.M.F.-C.).
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje