Tuning Nanopore Diameter of Titanium Surfaces to Improve Human Gingival Fibroblast Response
Autor: | Maria Del Mar Ferrà-Cañellas, Joana M. Ramis, Marta Monjo, Maria Antonia Llopis-Grimalt |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Scanning electron microscope
medicine.medical_treatment humanos Gingiva propiedades de superficie 02 engineering and technology Electrolyte lcsh:Chemistry Contact angle Nanopores 0302 clinical medicine Materials Testing Dental implant lcsh:QH301-705.5 Spectroscopy Cells Cultured Titanium Nanoporous General Medicine titanio 021001 nanoscience & nanotechnology Computer Science Applications soft tissue integration Nanopore encía nanoporos 0210 nano-technology proliferación celular Materials science Surface Properties Cells electrochemical anodization chemistry.chemical_element adhesión celular Catalysis Article Inorganic Chemistry 03 medical and health sciences medicine Cell Adhesion Humans Physical and Theoretical Chemistry perfiles de expresión génica Molecular Biology Cell Proliferation Anodizing Gene Expression Profiling Organic Chemistry nanopore diameter 030206 dentistry surface area Fibroblasts análisis de materiales fibroblastos lcsh:Biology (General) lcsh:QD1-999 chemistry células Biomedical engineering |
Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 19, Iss 10, p 2881 (2018) Volume 19 Issue 10 |
ISSN: | 1422-0067 |
Popis: | The aim of this study was to determine the optimal nanopore diameter of titanium nanostructured surfaces to improve human gingival fibroblast (hGF) response, with the purpose of promoting gingiva integration to dental implant abutments. Two TiO2 nanoporous groups with different diameters (NP-S 48 nm and NP-B 74 nm) were grown on Ti foils using an organic electrolyte containing fluoride by electrochemical oxidation, varying the applied voltage and the interelectrode spacing. The surfaces were characterized by scanning electron microscope (SEM), atomic force microscopy (AFM), and contact angle. The hGF were cultured onto the different surfaces, and metabolic activity, cytotoxicity, cell adhesion, and gene expression were analyzed. Bigger porous diameters (NP-B) were obtained by increasing the voltage used during anodization. To obtain the smallest diameter (NP-S), apart from lowering the voltage, a lower interelectrode spacing was needed. The greatest surface area and number of peaks was found for NP-B, despite these samples not being the roughest as defined by R-a. NP-B had a better cellular response compared to NP-S. However, these effects had a significant dependence on the cell donor. In conclusion, nanoporous groups with a diameter in the range of 74 nm induce a better hGF response, which may be beneficial for an effective soft tissue integration around the implant. This research was funded by the Osteology Foundation (13-069), the Ministerio de Educacion Cultura y Deporte (contract to M.A.L.-G; FPU15/03412), the Instituto de Salud Carlos III (contract to J.M.R; CP16/00124), and the Ministerio de Empleo y Seguridad Social with the Sistema de Garantia Juvenil (contract to M.d.M.F.-C.). |
Databáze: | OpenAIRE |
Externí odkaz: | |
Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |