Fecundity of Cryptosporidium parvum is correlated with intracellular levels of the viral symbiont CPV
Autor: | Ronald Fayer, James Higgins, Celia O'Brien, James M. Trout, Cheryl A. Lancto, Kalmia E. Kniel, Mitchell S. Abrahamsen, Mark C. Jenkins, Juan E. Abrahante |
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Rok vydání: | 2008 |
Předmět: |
Cell Survival
animal diseases Molecular Sequence Data Cattle Diseases Cryptosporidiosis Virulence Virus Microbiology Apicomplexa Feces parasitic diseases Animals Parasite hosting RNA Messenger Symbiosis Parasite Egg Count Cryptosporidium parvum Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction Oocysts DNA Protozoan biology.organism_classification Fecundity Virology Fertility Infectious Diseases RNA Viral Protozoa Cattle Parasitology RNA Protozoan |
Zdroj: | International Journal for Parasitology. 38:1051-1055 |
ISSN: | 0020-7519 |
Popis: | Differences in the virulence and fecundity of Cryptosporidium parvum isolates have been observed by several researchers studying cryptosporidiosis. The purpose of the present study was to determine if there was a correlation between intracellular levels of the viral symbiont CPV in C. parvum and fecundity of two isolates of the parasite, namely C. parvum Beltsville (B) and C. parvum Iowa (I). Dairy calves infected with 10(6)C. parvum-B excreted 5-fold more oocysts compared with calves infected with the same number of C. parvum-I oocysts. The increased fecundity of the former strain was corroborated by semi-quantitative PCR assay of DNA isolated from cell cultures infected with either C. parvum-B or C. parvum-I. Quantitative reverse transcriptase-PCR analysis of viral RNA revealed a 3-fold greater number of CPV in C. parvum-B compared with C. parvum-I oocysts. These findings may indicate a role for CPV in fecundity and possibly virulence of C. parvum. |
Databáze: | OpenAIRE |
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