In vitro reconstitution of recognition and activation complexes between interleukin-6 and gp130
Autor: | Dar-chone Chow, Nguyen Pham Tl, Garcia Kc, Stefan Rose-John, Ho J |
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Rok vydání: | 2001 |
Předmět: |
Macromolecular Substances
Genetic Vectors Immunoglobulins Plasma protein binding Biochemistry Immunoglobulin D Epitope Antigens CD Extracellular Cytokine Receptor gp130 Humans Receptor Membrane Glycoproteins biology Sequence Homology Amino Acid Chemistry Interleukin-6 digestive oral and skin physiology Surface Plasmon Resonance Glycoprotein 130 Receptors Interleukin-6 biological factors Recombinant Proteins Cell biology Protein Structure Tertiary Kinetics biology.protein Cytokines biological phenomena cell phenomena and immunity Signal transduction Ultracentrifugation Intracellular Protein Binding Signal Transduction |
Zdroj: | Biochemistry. 40(25) |
ISSN: | 0006-2960 |
Popis: | Gp130 is a shared signal-transducing receptor for a family of four-helix cytokines, of which interleukin-6 is a prototypic member. IL-6-type cytokines activate gp130 to elicit downstream intracellular JAK/STAT signaling cascades through formation of hetero-oligomeric receptor complexes. Interleukin-6 must first complex with its specific alpha-receptor (Ralpha) in order to bind and activate gp130. We have dissected the extracellular activation pathway of human gp130 by human IL-6 through reconstitution of soluble complexes representing intermediate and final states in the hierarchical assembly of the IL-6/IL-6Ralpha/gp130 signaling complex. To isolate these hetero-complexes, we have applied a protein engineering strategy of covalently linking IL-6 to its Ralpha, which results in a "hyperactive" single-chain complex (hyper-IL-6) which we express in both Escherichia coli and insect cells. We have determined that IL-6/IL-Ralpha and the cytokine-binding homology region (CHR) of gp130 (D2D3) form a stable trimolecular "recognition" complex (trimer) consisting of 1IL-6,1 IL-6Ralpha, and 1 gp130-CHR. Addition of the N-terminal (D1) Ig-like domain (IGD) of gp130 to the CHR results in a transition to a hexameric "activation" complex containing 2 IL-6, 2IL-6Ralpha, and 2 gp130. These results clearly demonstrate that the recognition and activation complexes are disparate hetero-oligomeric molecular species linked by the recruitment of the gp130 IGD by the unique site III epitope present on all gp130-class cytokines. The results of these studies are relevant to other members of the IL-6 family of gp130-cytokines and address a longstanding question concerning the respective roles of the gp130 CHR and IGD in assembly of the active signaling oligomer. |
Databáze: | OpenAIRE |
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