Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma
| Autor: | Camila Malta Romano, Cláudio Sérgio Pannuti, Karim Yaqub Ibrahim, Jan Felix Drexler, Adriana Fumie Tateno, Expedito José de Albuquerque Luna, L M Sumita, Cristiane Mendes de Oliveira, Paulo Henrique Braz-Silva, Ligia Camara Pierrotti, Tania Regina Tozetto-Mendoza, Maria Carmem Arroyo Sanchez |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Genes Viral genotype ORF K1 viruses AIDS-Related Opportunistic Infections Observational Study Real-Time Polymerase Chain Reaction SALIVA law.invention Open Reading Frames Viral Proteins 03 medical and health sciences 0302 clinical medicine law Genetic variation Genotype Humans Medicine 030212 general & internal medicine Genetic variability Sarcoma Kaposi Genotyping Phylogeny HHV-8 Polymerase chain reaction Acquired Immunodeficiency Syndrome saliva Molecular epidemiology business.industry Genetic Variation AIDS-KS virus diseases General Medicine Prognosis Virology Cross-Sectional Studies 030104 developmental biology Real-time polymerase chain reaction DNA Viral Immunology Female business Brazil Research Article |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Medicine |
| ISSN: | 0025-7974 |
| DOI: | 10.1097/md.0000000000005291 |
| Popis: | AIDS-associated Kaposi's sarcoma (AIDS-KS) caused by human herpes virus 8 (HHV-8) is the most severe and resistant form of KS tumor. Our aim was to verify whether there is an association between HHV-8 variability and development of AIDS-KS in Brazil by comparing the HHV-8 variability between individuals without and with KS. Saliva samples and blood, when available, were analyzed by polymerase chain reaction (PCR) techniques for detection of the fragments of ORF K1 of HHV-8, which were then genotyped and analyzed regarding the genetic variability. Our study described 106 positive cases for HHV-8 in the saliva from 751 AIDS patients without previous KS. In addition, we performed a phylogenetic analysis of HHV-8 in 34 of the 106 AIDS patients without KS and in 33 of the 37 patients with active KS. The distribution of HHV-8 genotypes A, B, C, and F in AIDS individuals was indistinguishable by comparing non-KS and KS groups, as well as regarding ethnicity. Considering the KS group, genotype B was associated with better prognosis of KS tumor. Interestingly, we found a particular profile of diversity within clade C and 2 recombinant patterns of HHV-8 in the saliva of AIDS individuals without KS. We emphasize the need to achieve standard genotyping protocol for ORF K1 amplification, thus allowing for substantial detection of HHV-8 variants. Our findings can shed light on the role of HHV-8 variability in the pathogenesis of AIDS-KS. |
| Databáze: | OpenAIRE |
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