C-terminal domains of general regulatory factors ABF1p and RAP1p in Saccharomyces cerevisiae display functional similarity
| Autor: | Paula Gonçalves, Rudi J. Planta, Karin Bergkamp‐Steffens, Kick Maurer, Willem H. Mager, Geerten P. van Nieuw Amerongen |
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| Přispěvatelé: | Chemistry and Pharmaceutical Sciences, Biochemistry and Molecular Biology, Molecular and Computational Toxicology |
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Transcriptional Activation
Saccharomyces cerevisiae Proteins Transcription Genetic Recombinant Fusion Proteins Saccharomyces cerevisiae Molecular Sequence Data Computational biology Microbiology Fungal Proteins Bacterial Proteins Transcription (biology) Genes Reporter Escherichia coli Amino Acid Sequence Binding site Promoter Regions Genetic Molecular Biology Gene Conserved Sequence DNA Primers Sequence Deletion Genetics chemistry.chemical_classification Reporter gene Binding Sites biology Base Sequence Serine Endopeptidases biology.organism_classification Fusion protein Amino acid DNA-Binding Proteins chemistry Rap1 Transcription Factors |
| Zdroj: | Conçalves, P M, Maurer, K, van Amerongen, G, Bergkamp-Steffens, K, Mager, W H & Planta, R J 1996, ' C-terminal domains of general regulatory factors ABF1p and RAP1p in Saccharomyces cerevisiae display functional similarity. ', Molecular Microbiology, vol. 19, pp. 535-543 . https://doi.org/10.1046/j.1365-2958.1996.404939.x CIÊNCIAVITAE Molecular Microbiology, 19, 535-543. Wiley-Blackwell Scopus-Elsevier |
| ISSN: | 0950-382X |
| Popis: | Abf1p and Rap1p are global regulatory factors which play an essential role in the transcription activation of yeast ribosomal protein genes. This functional link prompted us to investigate whether these factors may be functionally interchangeable. We focused on the indispensable C-terminal portions of both factors and performed mutual domain swaps. The functional capacity of the resulting hybrid proteins was subsequently examined using yeast strains conditionally expressing either the ABF1 or the RAP1 gene. Both the Abf1p–Rap1p and the Rap1p–Abf1p fusion proteins were found to be able to complement the growth defect of the respective strains. Furthermore, Abf1p and Rap1p are both able to promote transcription of a reporter gene through a combination of the respective binding site and a T-rich promoter element. These data strongly suggest that the C-terminal domains of Abf1p and Rap1p have, at least partially, identical functions. Finally, a deletion analysis of the so far largely uncharacterized C-terminal domain of Abf1p was performed, which revealed that two regions of 50 amino acids can perform all essential Abf1p functions. |
| Databáze: | OpenAIRE |
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