Improved Angiogenesis in Response to Localized Delivery of Macrophage-Recruiting Molecules
| Autor: | Tegy J. Vadakkan, Saniya Ali, Mary E. Dickinson, Jennifer L. West, Nejla Yosef, Chih-Wei Hsu, Larry D. Scott, Gisele A. Calderon, Jennifer E. Saik, Shang Wang, Ross A. Poché, Irina V. Larina |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Vascular Endothelial Growth Factor A
Angiogenesis Becaplermin lcsh:Medicine Neovascularization Physiologic Biology Neovascularization Cornea 03 medical and health sciences Mice 0302 clinical medicine Tissue engineering In vivo Cell Movement medicine Human Umbilical Vein Endothelial Cells Animals Humans lcsh:Science Cells Cultured 030304 developmental biology 0303 health sciences Multidisciplinary Tissue Engineering Tissue Scaffolds Macrophages lcsh:R Endothelial Cells Hydrogels Proto-Oncogene Proteins c-sis Cell biology Vascular endothelial growth factor A Self-healing hydrogels Immunology lcsh:Q Angiogenesis Inducing Agents Fibroblast Growth Factor 2 medicine.symptom 030217 neurology & neurosurgery Intravital microscopy Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 7, p e0131643 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Successful engineering of complex organs requires improved methods to promote rapid and stable vascularization of artificial tissue scaffolds. Toward this goal, tissue engineering strategies utilize the release of pro-angiogenic growth factors, alone or in combination, from biomaterials to induce angiogenesis. In this study we have used intravital microscopy to define key, dynamic cellular changes induced by the release of pro-angiogenic factors from polyethylene glycol diacrylate hydrogels transplanted in vivo. Our data show robust macrophage recruitment when the potent and synergistic angiogenic factors, PDGFBB and FGF2 were used as compared with VEGF alone and intravital imaging suggested roles for macrophages in endothelial tip cell migration and anastomosis, as well as pericyte-like behavior. Further data from in vivo experiments show that delivery of CSF1 with VEGF can dramatically improve the poor angiogenic response seen with VEGF alone. These studies show that incorporating macrophage-recruiting factors into the design of pro-angiogenic biomaterial scaffolds is a key strategy likely to be necessary for stable vascularization and survival of implanted artificial tissues. |
| Databáze: | OpenAIRE |
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